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pPLUS® AAV-Helper is a novel plasmid helper designed for AAV production. This plasmid has been optimized for triple transfection of HEK293 cells in suspension. The use of pPLUS® AAV-Helper leads to enhanced AAV titers and improved AAV particles quality.

Amount of Reagent
Item No.: 
101000182
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pPLUS®AAV-Helper
pPLUS®AAV-Helper

pPLUS® AAV-Helper is a novel plasmid helper designed for AAV production. This plasmid has been optimized for triple transfection of HEK293 cells in suspension. The use of pPLUS® AAV-Helper leads to enhanced AAV titers and improved AAV particles quality.

Item No.:
101000182

Overview

Specifications

This plasmid is the outcome of an extensive R&D program based on our proprietary DNA assembly technology (the eZyvec® technology) aiming at exploring the synergies of multiple genetic features to improve the infectivity (measured in TU/mL) and quality (full/empty ratio) of the viral particles obtained through triple transfection of HEK293 cells in suspension with FectoVIR®-AAV. They are readily available as an off-the-shelf product.

Key Benefits:

    Optimized for AAV Production with FectoVIR®-AAV: Increased AAV titers and AAV particles quality when used together with FectoVIR®-AAV
    Versatile: Suitable for use with various cell lines and production of several AAV serotypes.
    Compatible: With PEIpro® and other transfection reagents, suitable for use with various cell lines and production of several AAV serotypes.
    Adapted for your Projects from Research to Large Scale Manufacturing: Three packsize of Research Grade available, HQ and GMP Grade. GMP Grade coming soon (2025).

General Specifications

  • Amount of Reagent
    pPLUS® AAV-Helper 10 mg