CIMmultus® C4 HLD 800 mL cGMP Compliant Monolithic Column (6 μm)
CIMmultus® C4 HLD employs a high density of butyl ligands to achieve strong surface hydrophobicity. Relying on hydrophobic interaction chromatography (HIC), this column is a must for nucleic acid separations, providing an excellent solution for the purification of pDNA and RNA.
- Item No.:
- BIA-921.8136-6
*Custom/bulk order quotes are provided within 72 hours of request.
Choose your CIMmultus®
CIMmultus® is a unique and industry-proven line of pre-packed chromatographic monolithic columns featuring large flow-through channels, designed for working with large biomolecules such as viruses (AAV, adenovirus, lentivirus, influenza, bacteriophages), nucleic acids (mRNA, pDNA), virus-like particles and exosomes. CIMmultus® columns can be used as single-use disposable or multi-use columns. They are available in a variety of volume ranges – from 1mL for screening and method development purposes to 8000mL for industrial manufacturing.
CIMmultus® C4 HLD employs a high density of butyl ligands to achieve strong surface hydrophobicity. Relying on hydrophobic interaction chromatography (HIC), this column is a must for nucleic acid separations, providing an excellent solution for the purification of pDNA and RNA.
C4 (butyl) high ligand density (HLD) features:
- Strongly hydrophobic, uncharged
- Working range, pH 2–10
- Highly effective for removing proteins from nucleic acids (pDNA, RNA)
Your benefits:
- Very fast processes by convective mass transport
- Resolution independent of flow
- Ready to use columns
- High-capacity for large molecules due to fully accessible void volume in the channels
- Laminar flow minimizes shear stress resulting in high yields
Applications
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ApplicationPreparative Applications
Connections (Physical)
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Connector(s)TC 1" (25 mm), 8 mm ID bore
Dimensions
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Channel Size6 µm
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Column Tube Length150 mm
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Inner Diameter65 mm
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Outer Diameter105 mm
Features
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ReusableYes
General Specifications
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Flow Rate80–6400 mL/min | 12–977 cm/h | 0.1–8 CV/min
Materials of Construction
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Materials of ConstructionPoly(butyl methacrylate -co- ethylene dimethacrylate)
Operating Conditions
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Maximum Operating Pressure1.0 MPa | 10 bar | 145 psi
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Recommended Operating Temperature4 °C (39 °F) to 40 °C (104 °F)
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pH StabilityWorking range 3–13, cleaning in place 1–14
Physicochemical Information
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Bed Volume800 mL
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LigandC4 HLD (hydrophobic, butyl – high ligand density)
Product Information
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BrandCIMmultus®
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Package TypeIndividually Packed