Sartobind® Q strong ion exchangers are perfect tools for the polishing of monoclonal antibodies for the removal of viruses, DNA, host cell proteins, leached Protein A, and endotoxins. As size exclusion effects are neglectible, they are suited for purification of viruses such as adeno or lentiviruses displaying one order of magnitude higher dynamic binding capacity.

Pack Size
Item No.: 
99IEXQ42GC-----V
Availability: 

*Custom/bulk order quotes are provided within 72 hours of request.

Sartobind® Q membrane adsorbers display a macro-porous structure with pore size of > 3 µm. Quaternary ammonium ligands are bound covalently to the complete internal surface of the membrane, resulting in separation media of high binding capacity combined with exceptional high flow rates.

Applications virus and VLP capture:

As size exclusion effects are negligible, they are suited for purification of viruses and display one order of magnitude higher dynamic binding capacity compared to resin. Viruses such as adeno- or lentiviruses are captured at high flow rates. The material allows also >100 cycles of re-use without loss of binding capacity.

Benefits capture:

  • High speed purification at 5 - 30 bed volumes per minute
  • No size exclusion effects for large proteins an viruses / VLPs
  • >10fold higher binding capacities compared to conventional resins

Applications flow-through polishing:

Sartobind Q is the perfect tool for the flow-through polishing of valuable proteins such as monoclonal antibodies. The bed volumes can be kept sufficiently small during the removal of viruses, DNA, host cell proteins, leached Protein A, and endotoxins from such pharmaceutical proteins.

Benefits flow-through polishing:

  • Virus clearance > 6 log
  • DNA below detection limit
  • Host cell protein removal > 99 %
  • Endotoxin removal > 5 log
  • Save 80 % of column costs

Easy to use:

  • Plug and play devices from 0.08 mL to 5 L
  • Can be used like a filter
  • Flow rate up to 30 bed volumes/min
  • Re-usable or disposable

Applications

  • Application
    Screening

Connections (Physical)

  • Connector(s)
    N/A

Dimensions

  • Bed Height
    0.8 mm
  • Dimensions WxDxH
    128 × 85.5 × 25 mm
  • Membrane Area
    0.7 cm²

Features

  • Reusable
    No

Materials of Construction

  • Housing Material
    Polypropylene (PP)
  • Material 8-strip
    Polypropylene (PP)
  • Material Holding Frame
    Polystyrene
  • Materials of Construction
    Polyester
  • Membrane Material
    Stabilized reinforced cellulose

Operating Conditions

  • Maximum Operating Pressure
    35 kPa
  • pH Stability
    pH 2 – 14 (short term)

Physicochemical Information

  • Bed Volume
    19 µL/well
  • Ligand
    Quaternary ammonium
  • Ligand Density
    2 – 5 µeq/cm²
  • Pore Size
    3 – 5 µm
  • Reference Protein
    Bovine serum albumin (BSA)
  • Typical Dynamic Binding Capacity 10% per unit
    0.55 mg/well

Product Information

  • Brand
    Sartobind® Q
  • Membrane Adsorber Format
    96-Well Plates
  • Membrane Type
    Strong anion exchange membrane
  • Pack Size
    2
  • Type
    Membrane adsorber (96-well plate)
  • What you receive
    • 2 plates
    • 4 deep well collection plate 2 ml
    • 1 operating instruction

Sterility/Purity

  • Sterilization Method
    Autoclavable
  • Vivawell Vac96 Vacuum Manifold

    The Vac96 vacuum manifold has been designed specifically for use with Sartobind® 96 multiwell plates. The long drip nozzles on the outlet eliminate cross-talk between flow channels.