CIMmultus® C4 HLD 800 mL cGMP Compliant Monolithic Column (6 μm)

CIMmultus® C4 HLD employs a high density of butyl ligands to achieve strong surface hydrophobicity. Relying on hydrophobic interaction chromatography (HIC), this column is a must for nucleic acid separations, providing an excellent solution for the purification of pDNA and RNA.

Item No.: 
BIA-921.8136-6
Availability: 

*Custom/bulk order quotes are provided within 72 hours of request.

CIMmultus® is a unique and industry-proven line of pre-packed chromatographic monolithic columns featuring large flow-through channels, designed for working with large biomolecules such as viruses (AAV, adenovirus, lentivirus, influenza, bacteriophages), nucleic acids (mRNA, pDNA), virus-like particles and exosomes. CIMmultus® columns can be used as single-use disposable or multi-use columns. They are available in a variety of volume ranges – from 1mL for screening and method development purposes to 8000mL for industrial manufacturing.

CIMmultus® C4 HLD employs a high density of butyl ligands to achieve strong surface hydrophobicity. Relying on hydrophobic interaction chromatography (HIC), this column is a must for nucleic acid separations, providing an excellent solution for the purification of pDNA and RNA.

C4 (butyl) high ligand density (HLD) features:

  • Strongly hydrophobic, uncharged
  • Working range, pH 2–10
  • Highly effective for removing proteins from nucleic acids (pDNA, RNA)

Your benefits:

  • Very fast processes by convective mass transport
  • Resolution independent of flow
  • Ready to use columns
  • High-capacity for large molecules due to fully accessible void volume in the channels
  • Laminar flow minimizes shear stress resulting in high yields

Applications

  • Application
    Preparative Applications

Connections (Physical)

  • Connector(s)
    TC 1" (25 mm), 8 mm ID bore

Dimensions

  • Channel Size
    6 µm
  • Column Tube Length
    150 mm
  • Inner Diameter
    65 mm
  • Outer Diameter
    105 mm

Features

  • Reusable
    Yes

General Specifications

  • Flow Rate
    80–6400 mL/min | 12–977 cm/h | 0.1–8 CV/min

Materials of Construction

  • Materials of Construction
    Poly(butyl methacrylate -co- ethylene dimethacrylate)

Operating Conditions

  • Maximum Operating Pressure
    1.0 MPa | 10 bar | 145 psi
  • Recommended Operating Temperature
    4 °C (39 °F) to 40 °C (104 °F)
  • pH Stability
    Working range 3–13, cleaning in place 1–14

Physicochemical Information

  • Bed Volume
    800 mL
  • Ligand
    C4 HLD (hydrophobic, butyl – high ligand density)

Product Information

  • Brand
    CIMmultus®
  • Package Type
    Individually Packed