PULSin® Protein Delivery
The delivery of protein and antibody using PULSin® represents a powerful approach for functional studies. With PULSin® you can target intracellular proteins with antibodies in living cells without a fixation step, as well as study the function of a protein by controlling the expression level and the expression time course.
Amount of Reagent
- Price (USD):
- $513.00
- Item No.:
- 101000010
- Availability:
- pcs
Choose your Transfection Reagent For Life Science Research
PULSin® is composed of a proprietary cationic amphiphile molecule that forms non-covalent complexes with proteins and antibodies. Once formed, complexes are internalized via anionic cell-adhesion receptors and are released into the cytoplasm where they disassemble. The process is non-toxic and delivers functional proteins.Key Benefits:
- Unique: One reagent for protein, antibody, and peptide delivery into mammalian cells.
- Optimized: Efficient delivery in live primary cells and difficult to transfect cells.
- Innovative: Easy to use alternative to chemical conjugation and viral transduction
Applications
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Cell TypeAdherent & suspension cells grown in presence of serum
Biological Information
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Hepes dilution buffer20 mL
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Molecule deliveredProtein, Antibody, Peptide
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Number of transfections0.4 ml of PULSin® delivery reagent is sufficient to perform 25 delivery experiments in 6-well plates and 100 experiments in 24-well plates
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Positive control (R-phycoerythrin)20 µg
General Specifications
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Amount of ReagentPULSin® 0.4 mL
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Reagents and Kits TypePULSin® - Protein delivery
Product Information
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Provided WithHepes Buffer (20mM) for protein dilution, R-Phycoerythrin used as a positive control
Storage Conditions
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Storage Conditions5 °C ± 3 °C