Microsart® Calibration Reagent Clostridium sporogenes
Choose your Microsart® Rapid Sterility Testing
Microsart® Calibration Reagents provide isolated genomic DNA of a specific bacterial species. Titrated genomic DNA can serve for creating standard curves by using dilutions of the material as sample in a quantitative Real-time PCR. Furthermore quantified genomic DNA can be used for specifity testing during assay validation.
To obtain the microorganisms, a liquid culture medium was inoculated with an early passage strain and harvested at the end of the logarithmic growth phase by repeated washing and centrifugation. After DNA extraction and purification the concentration of the DNA was quantified photometrically (A 260/280) and with an ultrasensitive fluorescent nucleic acid stain for quantitating double-stranded DNA (dsDNA) calibrated to weight reference standards and controlled by qPCR (compared against exactly quantified calibrator plasmids).
Applications
-
ApplicationBacteria Detection
-
Application AreaRapid testing
Biological Information
-
SpeciesClostridium sporogenes
Components
-
Additional Required Components
-
qPCR cycler
-
PCR reaction tubes
-
Centrifuge for 1.5 mL PCR tubes
-
Pipettes
- PCR kit
-
qPCR cycler
Dimensions
-
Filter DiameterN/A
General Specifications
-
Culture MediaN/A
-
Detection MethodPCR
-
Membrane Color | Grid ColorN/A
Materials of Construction
-
Filter MaterialN/A
Physicochemical Information
-
Pore SizeN/A
Product Information
-
BrandMicrosart®
-
Pack SizeN/A
-
TypeReagents
-
What you receive
-
1 vial with 10^8 genome copies lyophilized
- 3 vials buffer
-
1 vial with 10^8 genome copies lyophilized
Share Your Thoughts!
Help us amplify the customer experience by providing a review of your recently purchased Sartorius product.