Octet® Protein L (ProL) Biosensors

Octet^® ProL Biosensors are coated with recombinant Protein L, which recognizes a wider range of antibody classes than both Protein A and Protein G, including IgG, IgM, IgA, IgE and IgD. These biosensors offer a rapid and direct method for quantitation of a broad set of kappa light chain containing IgG proteins, including whole molecules, FAb fragments and single chain variable fragments. Octet^® Protein L (ProL) Biosensors are especially useful for quantifying antibodies and antibody fragments from serum-based cultures because Protein L does not bind bovine immunoglobulins, which often contaminate serum supplements.

(Note that Protein L does not recognize all kappa light chains. For example, Protein L recognizes mouse VKl (but not VKll, VKIII and VKIV) and human VKI, VKIII and VKIV (but not VKII).)

Key Specifications

• Typical dynamic range for Fab: 0.05 - 2000 µg/mL
• Sensitivity can be extended by longer read times and higher shake speed
• Throughput: 16 and 96 samples in as little as 2 minutes on Octet^® RH16 and RH96 respectively
• Precision: CV < 10%

Cost-Effective Regeneration

Octet^® ProL Biosensors can be regenerated up to 10 times via a standard low pH protocol allowing for additional analyses and providing a cost-effective format for analyzing large sample libraries.