Incucyte® MMP Reagent Kit

The MMP Orange Reagent Kit assesses changes in MMP, an indicator of mitochondrial health. The cell-permeable MMP Orange Reagent accumulates in active mitochondria in proportion to the MMP generated by the electrochemical gradient across the mitochondrial membrane. A drop in MMP denotes a disruption in mitochondrial function and/or integrity and can also be used as an early indicator of apoptosis.

This assay kit utilizes two control compounds: FCCP, an uncoupling agent that transports H+ ions across the mitochondrial membrane and results in depolarization (indicated by a decrease in fluorescence), and Oligomycin A, an inhibitor of ATP synthase which results in hyperpolarization (indicated by an increase in fluorescence). These reagents have been validated for use with the Incucyte^® Live-Cell Analysis System configured with an SX5 Metabolism Optical Module, Green/Orange/NIR or Orange/NIR Optical Module and enables the real-time evaluation of mitochondrial membrane potential.

The Incucyte MMP Orange Reagent Kit is intended for use with the Incucyte^® Cell-by-Cell Analysis Software Module (Cat. No. 9600-0031).