Polyamine Analysis by UHPLC-MS/MS

Polyamine levels in mammalian cells play a key role in cells’ viability as well as protein synthesis. Knowing the polyamine content is a powerful tool for boosting efficiency and production. Moreover, they play a key role in protein and RNA production and are involved in different metabolic steps in the human and animal body. Most polyamines need to be acquired from nutrition, which makes it essential to determine the polyamine content in food and feed samples.

Our method is UHPLC-MS/MS based and therefore offers quantitative results with low LOQs for the following polyamines: Putrescine 0.033[mg/L], Spermidine 0.033 [mg/L], Spermine 0.1 [mg/L].

Deliverables: We will send you a short report summarizing your data within 5 business days (After receipt of samples and if no additional sample preparation / matrix evaluation is necessary. Valid for sets of more than 5-50 samples).

Sample Requirements: Usually a sample amount of 1 mg (if purity is > 80%) or 0.5 ml is needed for each analytical service. If you are limited in your sample amount, please contact us. We are open to discuss smaller volumes. If you send frozen samples for more than one analysis, it would be desirable to ship them in aliquots, to prevent repeated freezing and thawing.

Samples can be spent cell culture media or cell culture supernatants. Please read and sign our sample declaration sheet. Prepare and ship your samples according to our sample preparation protocol.

Our methods are adapted to state-of-the-art cell culture media.