CIMmultus® OH 400 mL cGMP Compliant Monolithic Column (2 µm)

CIMmultus^® Monoliths are reusable chromatography devices for scalable high-resolution purification of complex biological samples. Inside the custom designed housing is a single piece stationary phase with homogeneous channel size and surface chemistry. This stationary phase is designed to simplify method transfer from development into production.

CIMmultus^® columns can be used as single-use disposable or multi-use columns. They are available in a variety of volume ranges – from 1mL for screening and method development purposes up to 40L for industrial manufacturing.

CIMmultus^® OH relies on hydrophobic interaction chromatography (HIC) to provide an excellent solution for the purification of large biomolecules, such as virus particles, VLPs and extracellular vesicles.

OH (hydroxy) features:

• Uncharged (neutral) ligand
• Working range, pH 2–10
• Retains very large solutes (virus particles, VLP, vesicles) in the presence of precipitating salts or polyethylene glycol, and elutes them with high resolution in order of increasing size
• Can be used as a pre-capture step to reduce most host cell impurities in AAV purification
• Elution with descending salt or polyethylene glycol
• Sanitizable with 1 M NaOH

Benefits:

• Very fast processes by convective mass transport – resolution independent of flow
• Ready to use columns
• High-capacity for large molecules due to fully accessible void volume in the channels
• Laminar flow minimizes shear stress resulting in high yields