CIMmultus® Oligo dT18 (C12 Linker) 400 mL Monolithic Column (2 μm)

This high-performance column is primarily intended for capturing ssRNA with a poly-adenylated tail, making it an excellent tool for the purification of mRNA in IVT reaction mixtures.

Item No.: 
814.1219-2
Availability: 
Piece
1

*Custom/bulk order quotes are provided within 72 hours of request.

CIMmultus® Monoliths are reusable chromatography devices for scalable high-resolution purification of complex biological samples. Inside the custom designed housing is a single piece stationary phase with homogeneous channel size and surface chemistry. This stationary phase is designed to simplify method transfer from development into production.

They can be used as single-use disposable or multi-use columns, and they are available in various volume ranges – from 1mL for screening and method development purposes up to 40L for industrial manufacturing.

The ligand in CIMmultus® Oligo dT18 is an 18-mer deoxythymidine chain covalently immobilised on the monolith with a carbon linker. RNA with a poly(A) tail binds on the ligand by hybridisation between adenine (A) bases on the RNA tail and deoxythymine (T) bases of the ligand. Species that do not contain a poly(A) tail do not bind on the column and are found in the unbound (flow-through) fraction.

Oligo dT (deoxythymine) features:

  • Oligo dT ligands covalently bound to a solid support
  • Hybridises to the poly-adenylated tail which is present on most eukaryotic mRNAs, or synthetised onto the molecule during IVT
  • Primarily intended for fast and efficient capture and purification of mRNA with a poly-adenylated tail, from various sources
  • Can selectively remove impurities from the sample
  • Convenient approach for initial purification of mRNA

Benefits:

  • Fewer unit operations: apply IVT material to monolith without buffer exchange
  • Very fast processes by convective mass transport – resolution independent of flow
  • Ready to use columns
  • High-capacity for large molecules due to fully accessible void volume in the channels
  • Laminar flow minimizes shear stress resulting in high yields

Applications

  • Application
    Preparative Applications

Connections (Physical)

  • Connector(s)
    TC 1" (25 mm), 8 mm ID bore

Dimensions

  • Channel Size
    2 µm
  • Column Tube Length
    75 mm
  • Inner Diameter
    65 mm
  • Outer Diameter
    105 mm

Features

  • Reusable
    Yes

General Specifications

  • Flow Rate
    40–800 mL/min | 12–245 cm/h | 0.1–2 CV/min

Materials of Construction

  • Materials of Construction
    Poly(glycidyl methacrylate -co- ethylene dimethacrylate)

Operating Conditions

  • Maximum Operating Pressure
    1.4 MPa | 14 bar | 200 psi
  • Recommended Operating Temperature
    4 °C (39 °F) to 30 °C (86 °F)
  • pH Stability
    Linkage stability: pH 2–13. Ligand stability not tested. Functionality retained after exposure to 0.1 M NaOH for a few hours.

Physicochemical Information

  • Bed Volume
    400 mL
  • Ligand
    Oligo dT18 coupled to CDI-activated matrix, C12 Linker
  • Ligand Density
    > 0.5 mg Oligo dT/mL wet support

Product Information

  • Brand
    CIMmultus®
  • Package Type
    Individually Packed