CIMmultus® Oligo dT18 (C12 Linker) 400 mL Monolithic Column (2 μm)
This high-performance column is primarily intended for capturing ssRNA with a poly-adenylated tail, making it an excellent tool for the purification of mRNA in IVT reaction mixtures.
- Item No.:
- 814.1219-2
- Piece
- 1
*Custom/bulk order quotes are provided within 72 hours of request.
Choose your CIMmultus®
CIMmultus® Monoliths are reusable chromatography devices for scalable high-resolution purification of complex biological samples. Inside the custom designed housing is a single piece stationary phase with homogeneous channel size and surface chemistry. This stationary phase is designed to simplify method transfer from development into production.
They can be used as single-use disposable or multi-use columns, and they are available in various volume ranges – from 1mL for screening and method development purposes up to 40L for industrial manufacturing.
The ligand in CIMmultus® Oligo dT18 is an 18-mer deoxythymidine chain covalently immobilised on the monolith with a carbon linker. RNA with a poly(A) tail binds on the ligand by hybridisation between adenine (A) bases on the RNA tail and deoxythymine (T) bases of the ligand. Species that do not contain a poly(A) tail do not bind on the column and are found in the unbound (flow-through) fraction.
Oligo dT (deoxythymine) features:
- Oligo dT ligands covalently bound to a solid support
- Hybridises to the poly-adenylated tail which is present on most eukaryotic mRNAs, or synthetised onto the molecule during IVT
- Primarily intended for fast and efficient capture and purification of mRNA with a poly-adenylated tail, from various sources
- Can selectively remove impurities from the sample
- Convenient approach for initial purification of mRNA
Benefits:
- Fewer unit operations: apply IVT material to monolith without buffer exchange
- Very fast processes by convective mass transport – resolution independent of flow
- Ready to use columns
- High-capacity for large molecules due to fully accessible void volume in the channels
- Laminar flow minimizes shear stress resulting in high yields
Applications
-
ApplicationPreparative Applications
Connections (Physical)
-
Connector(s)TC 1" (25 mm), 8 mm ID bore
Dimensions
-
Channel Size2 µm
-
Column Tube Length75 mm
-
Inner Diameter65 mm
-
Outer Diameter105 mm
Features
-
ReusableYes
General Specifications
-
Flow Rate40–800 mL/min | 12–245 cm/h | 0.1–2 CV/min
Materials of Construction
-
Materials of ConstructionPoly(glycidyl methacrylate -co- ethylene dimethacrylate)
Operating Conditions
-
Maximum Operating Pressure1.4 MPa | 14 bar | 200 psi
-
Recommended Operating Temperature4 °C (39 °F) to 30 °C (86 °F)
-
pH StabilityLinkage stability: pH 2–13. Ligand stability not tested. Functionality retained after exposure to 0.1 M NaOH for a few hours.
Physicochemical Information
-
Bed Volume400 mL
-
LigandOligo dT18 coupled to CDI-activated matrix, C12 Linker
-
Ligand Density> 0.5 mg Oligo dT/mL wet support
Product Information
-
BrandCIMmultus®
-
Package TypeIndividually Packed