NutriFreez® D10 Cryopreservation Medium

NutriFreez^® D10 Cryopreservation Medium is a ready-to-use, animal component-free, serum-free, and protein-free cell freezing solution optimized for multiple cell lines. When culturing cells in a serum-free environment, it is essential to also maintain serum-free conditions during cryopreservation.

NutriFreez^® D10 Cryopreservation Medium has been demonstrated to result in high rates of cell viability, proliferation, adherence (in relevant lines), and bioactivity/ expression following freezing and thawing. Superior results were obtained both in comparison with serum-containing freezing media as well as competing serum-free products, making this an ideal product for both serum-containing and serum-free applications.

NutriFreez^® D10 Cryopreservation Medium has been extensively tested on multiple cell types, including:

• Human Mesenchymal Stem Cells (hMSC), from various sources (e.g. bone marrow (BM-MSC), adipose tissue (AT-MSC), umbilical cord tissue (UC-MSC) derived and dental pulp tissue (hMSC-DP)
• Human Embryonic Stem Cells (hESC), Induced Pluripotent Stem Cells (iPSC)
• Human Peripheral Blood Mononuclear Cells (PBMC's)
• Human Endothelial Cells (EC)
• T cells, including Chimeric Antigen Receptors (CART) cells and Tumor-Infiltrating Lymphocytes (TIL's)
• Neuron Cells
• Hybridomas
• CHO cells
• Vero Cells
• Multiple mammalian cell lines: MRC-5, HEK-293, HepG2, HeLaBSC-1, BGM3T3, MA-10BHK-21 as well as other extremely sensitive cell types

NutriFreez^® D10 Cryopreservation Medium Features

• Cryoprotective formulation designed to minimize dehydration effects
• Effective maintenance of cell viability, adhesion, and bioactivity
• Ready-to-use solution, simple protocol
• Suitable for use in cell banks and biopharmaceutical applications
• Animal component-free, serum-free, protein-free solution
• Manufactured in accordance with applicable cGMP guidelines
• Drug Master File available

Results of a cryopreservation comparison performed at the Research and Development Center for Cell Therapy (Japan) on human pluripotent stem cells (hESC and hiPSC) show that NutriFreez^® D10 Cryopreservation Medium* presents best recovery rate for hPSCs after thawing (<a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4396159/" target="_blank">Nishishita et al. Am J Stem Cells. 2015)</a>.

Furthermore, an evaluation of different cryopreservation agents for mesenchymal stem cells (MSC) performed at Ottawa Hospital Research Institute (Canda) shows that MSC cryopreserved in NutriFreez^® D10 Cryopreservation Medium exhibit the best post-thaw viability (<a href="{{media url="wysiwyg/documents/Posters/ISCT-2018_poster_cryopreservant_comparison_study_Shirley_Mei_OHRI_1_.pdf"}}" target="blank">Salkhordeh et. al. Cytotherapy, May 2018</a>).

* NutriFreez^® brand replaces the brand CryoStem™