NutriFreez® D10 Cryopreservation Medium without Phenol Red
NutriFreez® D10 Cryopreservation Medium is a ready-to-use, animal component-free, serum-free, and protein-free cell freezing solution optimized for multiple cell lines. When culturing cells in a serum-free environment, it is essential to also maintain serum-free conditions during cryopreservation.
NutriFreez® D10 Cryopreservation Medium has been demonstrated to result in high rates of cell viability, proliferation, adherence (in relevant lines), and bioactivity/ expression following freezing and thawing. Superior results were obtained both in comparison with serum-containing freezing media as well as competing serum-free products, making this an ideal product for both serum-containing and serum-free applications.
NutriFreez® D10 Cryopreservation Medium has been extensively tested on multiple cell types, including:
- Human Mesenchymal Stem Cells (hMSC), from various sources (e.g. bone marrow (BM-MSC), adipose tissue (AT-MSC), umbilical cord tissue (UC-MSC) derived and dental pulp tissue (hMSC-DP)
- Human Embryonic Stem Cells (hESC), Induced Pluripotent Stem Cells (iPSC)
- Human Peripheral Blood Mononuclear Cells (PBMC's)
- Human Endothelial Cells (EC)
- T cells, including Chimeric Antigen Receptors (CART) cells and Tumor-Infiltrating Lymphocytes (TIL's)
- Neuron Cells
- Hybridomas
- CHO cells
- Vero Cells
- Multiple mammalian cell lines: MRC-5, HEK-293, HepG2, HeLaBSC-1, BGM3T3, MA-10BHK-21 as well as other extremely sensitive cell types
NutriFreez® D10 Cryopreservation Medium Features
- Cryoprotective formulation designed to minimize dehydration effects
- Effective maintenance of cell viability, adhesion, and bioactivity
- Ready-to-use solution, simple protocol
- Suitable for use in cell banks and biopharmaceutical applications
- Animal component-free, serum-free, protein-free solution
- Manufactured in accordance with applicable cGMP guidelines
- Drug Master File available
Applications
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Application AreaCryopreservation
Compliance Information
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Animal ComponentsNo
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Animal Origin (AO) / Non-Animal Origin (NAO)Non-Animal Origin (NAO)
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GMP ComplianceYes
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OriginAnimal-Component Free
Components
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Phenol Red IndicatorWithout Phenol Red
Features
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Serum FreeYes
Physicochemical Information
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Concentration10% DMSO
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FormulationMedia-based
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Protein FreeYes
Product Information
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BrandNutriFreez®
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Package TypeBottle
Sterility/Purity
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Delivery ConditionSterile
Volume
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Volume50 mL