PULSin® Protein Delivery

Tech Support Documents
PULSin® Protein Delivery
 

The delivery of protein and antibody using PULSin® represents a powerful approach for functional studies. With PULSin® you can target intracellular proteins with antibodies in living cells without a fixation step, as well as study the function of a protein by controlling the expression level and the expression time course.

Amount of Reagent
Price (USD):
$513.00
Item No.: 
101000010
Availability: 

*Custom/bulk order quotes are provided within 72 hours of request.

Capabilities

PULSin® enables delivery of protein irrespective of its size

PULSin® is able to deliver R-phycoerythrin, a fluorescent protein (240 kD), to the cytoplasm of up to 98% cells. The protein is evenly distributed in the cytoplasm and excluded from the nucleus due to its large size.

PULSin® efficiently delivers antibodies in live cells

Antibodies are successfully delivered in mammalian cells and able to recognize their target protein inside the cytoplasm. PULSin® enabled the delivery of FITC-labeled anti-alpha-tubulin to the cytoplasm of high percentage of HeLa cells (85%). Similarly, anti-giantin Alexa Fluor® 488 was delivered to the cytoplasm of 98% of live HeLa cells, labeling the Golgi apparatus.

Simple protocol for successful protein delivery.

PULSin® will save you time and efforts compared to other techniques using viral transduction or chemical conjugation. PULSin® reagent is ready-to-use and provided with a dilution buffer and a fluorescent control protein (R-phycoerythrin).
The protocol is fast: simply add the reagent to the protein, incubate and add to the cells. Cells can be analyzed starting 4 hours post-delivery.

Easy to use alternative to chemical conjugation and viral transduction.

Comparison of PULSin® with two other protein delivery reagents shows a higher efficiency of protein delivery, Moreover, the amount of protein delivered per cell is higher with PULSin® as measured for R-phycoerythrin protein and for FITC-alpha-tubulin antibody (cf additional data).

PULSin® is composed of a proprietary cationic amphiphile molecule that forms non-covalent complexes with proteins and antibodies. Once formed, complexes are internalized via anionic cell-adhesion receptors and are released into the cytoplasm where they disassemble. The process is non-toxic and delivers functional proteins.Key Benefits:

  • Unique: One reagent for protein, antibody, and peptide delivery into mammalian cells.
  • Optimized: Efficient delivery in live primary cells and difficult to transfect cells.
  • Innovative: Easy to use alternative to chemical conjugation and viral transduction

Applications

  • Cell Type
    Adherent & suspension cells grown in presence of serum

Biological Information

  • Hepes dilution buffer
    20 mL
  • Molecule delivered
    Protein, Antibody, Peptide
  • Number of transfections
    0.4 ml of PULSin® delivery reagent is sufficient to perform 25 delivery experiments in 6-well plates and 100 experiments in 24-well plates
  • Positive control (R-phycoerythrin)
    20 µg

General Specifications

  • Amount of Reagent
    PULSin® 0.4 mL
  • Reagents and Kits Type
    PULSin® - Protein delivery

Product Information

  • Provided With
    Hepes Buffer (20mM) for protein dilution, R-Phycoerythrin used as a positive control

Storage Conditions

  • Storage Conditions
    5 °C ± 3 °C