Incucyte® Caspase-3/7 Dye for Apoptosis

Name: Incucyte® Caspase-3/7 Dye for Apoptosis
Brand: Sartorius
SKU: 4776
Price: 402.0 USD
Availability: InStock

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One vial (20 µL) of mix-and-read reagent in DMSO sufficient for 100-200 live-cell apoptosis tests (1 test = 1 well of 96-well microtiter plate) in 96-well format.

Top Features of Incucyte^® Caspase-3/7 Dye for Apoptosis

Reduce loss of precious or compromised cells under-going caspase-3/7 mediated apoptosis with mix-and-read reagents
Track true, artifact-free biological responses with non-perturbing, live-cell reagents
Quantify apoptosis in real-time with the Incucyte^® Live-Cell Analysis System

Popular Applications for Incucyte^® Caspase-3/7 Dye for Apoptosis

Immune cell killing
Multiplex with proliferation
Multi-spheroid growth

Please note that all sales are final and that this product is not returnable, with the exception of warranty issues covered under Sartorius standard terms and conditions. If you have any issues with this product, please contact your Sartorius sales or service representative for assistance.

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Overview

Incucyte^® Caspase-3/7 Dyes for Apoptosis are formulated to detect apoptosis (cell death) in live cells. The dyes are ideally suited to the mix-and-read, real-time quantification of cells undergoing caspase-3/7 mediated apoptosis. Addition of the Incucyte^® Caspase-3/7 Green, Red or Metabolism Dyes to healthy cells is non-perturbing to cell growth and morphology. When used with Incucyte^® Live-Cell Analysis Systems and software, these reagents allow for reliable, accurate evaluation of cell death. Incucyte^® Caspase-3/7 Dyes for Apoptosis couple the activated caspase-3/7 recognition motif (DEVD) to a DNA intercalating dye. When added to tissue culture medium, the inert, non-fluorescent substrate crosses the cell membrane where it is cleaved by activated caspase-3/7, resulting in the release of the DNA dye and fluorescent staining of the nuclear DNA. With Incucyte^® integrated analysis software, fluorescent objects can be quantified and background fluorescence minimized. These reagents have been validated for use with the Incucyte^® Live-Cell Analysis System and enable real-time evaluation of cell apoptosis induced by pharmacological agents and/or genetic and environmental factors.

Presentation, Storage and Stability

The dyes are supplied as single-vial solutions in dimethyl sulfoxide (DMSO). Each vial provides a sufficient quantity for performing 100-200 tests (one test is one well of a 96-well microtiter plate). Upon receipt, the Incucyte^® Caspase 3/7 Dyes should be stored at 4°C where they are stable for six months.

Recommended Use

We recommend diluting Incucyte^® Caspase-3/7 Dyes in complete culture media and adding directly to cells in culture, as per the guidelines below:

Incucyte^® Caspase-3/7 Green: 1:1000 in culture media, for a final assay concentration of 5 μM
Incucyte^® Caspase-3/7 Red: Optimize final assay concentration by diluting the reagent 1:200 in complete medium, then make a series of 2-fold dilutions for final assay concentrations of 2.5, 1.25 and 0.5 µM
Incucyte^® Caspase-3/7 for Metabolism: 1:1000 in culture media, for a final assay concentration of 1 μM

Additional protocol details can be found in the Product Guide under the Documents tab.

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