CHO TF Medium, Powder prepares 10 L
Perfect medium for working with transient CHO cells - growth, transfection and production -
CHO TF is a complete chemically defined, animal-component-free cell culture medium. It was developed for cultivation of CHO and other mammalian cell lines, with a special focus on transfection applications and virus production. The medium is especially suited for transient transfection with e.g. polycationic transfection reagents such as polyethylenimine (PEI). CHO TF supports cell growth and production of recombinant proteins and antibodies in high-density suspension culture. It can be used in research or in manufacturing applications.
Volume
- Item No.:
- 886-0010DPM
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- Suitable for various CHO clones (e.g., DG44, K1, CHO S, CHO GS)
- Chemically defined, serum-free, animal component-free and hydrolysate-free
- Little or no adaptation required from other serum-free media
- Supports stable growth of suspension cells at high viability in seed train culture
- Compatibility with cationic transfection reagents (e.g. PEI) facilitates simplified transfection
- w/o L-glutamine
- w/o growth factor
- available as "SILAC" variant w/o arginine and lysine
- Approved by ISO 9001 QMS (Quality Statement)
User Manual
Applications
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ApplicationProtein
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Application AreaCHO cells
Compliance Information
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Animal Origin (AO) / Non-Animal Origin (NAO)NAO
Features
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Serum FreeYes
Physicochemical Information
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Adherence/SuspensionSuspension
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Chemically Defined (CD)Yes
Product Information
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Package TypeBucket
Storage Conditions
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Storage Conditions2 °C (36°F) to 8 °C (46°F), solution of ethanol and buffer (100 mM NaCl, 20 mM TRIS pH 7.5) in the ratio 1:4 (v/v)
Volume
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Volume10L