Sartobind STIC® PA Capsule 400 mL with 4 mm bed height
Choose your Sartobind STIC® PA
Sartobind STIC® PA removes contaminants at high salt. Salt-tolerant interaction chromatography (STIC) with PA ligand is based on anion-exchange chromatography (AEX) principles. AEX is the established method for removing process-derived contaminants such as host-cell proteins (HCP), DNA, endotoxins, and adventitious and endogenous viruses. It is performed during the downstream processing of monoclonal antibodies (mAbs) in flow-through mode. The process is driven by throughput and not by mass, and thus membrane chromatography (MC) is advantageous over columns for contaminant removal, as membranes run up to two orders of magnitude higher flow rates. Membranes are easy to operate and can be disposed of after one use, just like filters, to save on validation cost.
Applications:
Current mAb downstream processes consist of at least one initial capture and one AEX chromatography step. The limitation of AEX steps is often that the Q chemistry requires low-salt conditions. In processes with cation exchange (CEX) as the second chromatography step, the CEX pool needs to be diluted to conductivities of 4-7 mS/cm to reestablish binding conditions for the following AEX step. The Sartobind STIC PA membrane is composed of cross-linked, regenerated macro-porous cellulose. The primary amine ligand is attached to the matrix at approximately six-fold higher ligand density than that of Q membrane. Both the free amine and the high ligand density were crucial for developing its high salt tolerance.
Benefits:
The Sartobind STIC® adsorber speeds up the production process by high flow rate and opens new opportunities for the purification of therapeutic proteins and vaccines.
- No dilution of CEX pools necessary
- Host cell proteins up to 10kg/l (product load/liter membrane)
- Viruses >4 log
- DNA at up to 1.5 M NaCl
- Endotoxins >4 log
Easy to use:
- Plug and play devices from 0.08 ml to 1.6 L
- Can be used like a filter
- Flow rate up to 30 bed volumes/min
- Disposable after one use
Brochure
Datasheet
Flyer
User Manual
Applications
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ApplicationPolishing
Compliance Information
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Biological ReactivityAll materials of this filter element meet the requirements of the current USP Biological Reactivity tests <88> for plastics Class VI (Systemic Injection, Intracutaneous and Implantation tests).
Connections (Physical)
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Connector(s)Sanitary 1" – 1.5"
Dimensions
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Bed Height4 mm
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Dimensions WxDxH10 × 57 cm
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Membrane Area1.46 m²
Features
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ReusableNo
Materials of Construction
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Housing MaterialPolypropylene (PP)
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Materials of Construction
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Polypropylene
- Polyester
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Polypropylene
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Membrane MaterialStabilized reinforced cellulose
Operating Conditions
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Maximum Operating Pressure0.4 MPa
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Maximum Venting Pressure0.5 bar, 0.05 MPa, 7 psi
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Recommended Flow Rate8 L/min
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pH StabilitypH 2 – 14 (short term)
Physicochemical Information
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Bed Volume400 mL
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LigandPrimary amine (PA)
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Ligand Density18 – 22 µeq/cm²
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Pore Size3 – 5 µm
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Reference ProteinBovine serum albumin (BSA)
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Thermal StabilitySartobind capsules with ion exchange membranes can be autoclaved at 121 °C for 30 min. We recommend one autoclaving cycle only.
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Typical Dynamic Binding Capacity 10% per unit20 g
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Void Volume1.08 L
Product Information
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BrandSartobind® STIC PA
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Membrane Adsorber FormatCapsules
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Membrane TypeSalt tolerant interaction membrane (anion exchanger)
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Pack Size1
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TypeMembrane adsorber
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What you receive
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1 capsule
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1 operating instruction
- 1 certificate
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1 capsule