BioPAT® Trace Buffer Salt Mixture Glc/ Lac cell cult

BioPAT® Trace and BioPAT® Multi Trace require two calibration solutions and a transport buffer to function, and accurately determine the parameter concentration in a sampled vessel. These liquids are connected on the labeled end points of the Tube set and are automatically pumped within the system loop. The transport buffer and vessel solution do not directly mix. However, during the BioPAT® Trace sampling cycle the transport buffer flow is paused and the concentration gradient drives small molecules from the bulk into the transport buffer. When flow is resumes after a set time period, this accumulated concentration is transported to the biosensor for analysis. This data is then correlated to the linear calibration to yield the bulk concentration. This sampling method uses the diffusion of small molecules across the permeable membrane without any loss of vessel volume. During an analysis, the measurement line is flushed with a transport buffer, which is an acceptor stream that can be stopped from behind the membrane for a defined period of time. This procedure is carried out by triggering a valve, which in turn opens up a bypass route.