PPA HyperCel PRC Column - 5 mL

Tech Support Documents
PPA HyperCel PRC Column - 5 mL PPA HyperCel PRC Column - 5 mL
 

PPA HyperCel is a mixed-mode resin with an aromatic ligand exhibiting stronger hydrophobicity and providing a different selectivity in protein purification. Pre-packed columns simplify resin screening and selection, and are ideal for process step optimization. The RoboColumn format is especially suited to high throughput process development (HTPD).

Bed Volume
Price (USD):
$243.00
Item No.: 
PRC08X100PPAHCEL
Availability: 

*Custom/bulk order quotes are provided within 72 hours of request.

PPA HyperCel resin is an industry-scalable chromatography resin designed for protein capture and impurity removal in a biopharmaceutical environment. Operating on a “mixed-mode” mechanism, with a phenlypropylamine ligand, its behavior is based on a combination of electrostatic and hydrophobic interactions of the proteins with the ligands. PPA HyperCel resin provides unique selectivities, different from those given by ion exchange or conventional HIC (hydrophobic interaction chromatography).

Features and Benefits

- High mechanical and chemical stability
- Suitable for large-scale chromatography in a biopharmaceutical environment
- Cross-linked cellulose base matrix


Applications

- Protein purification at low ionic strength by direct hydrophobic capture
- Separation of challenging mixtures, e.g. protein isoforms or proteins with very close pIs
- Orthogonal separation mechanism to ion exchange or HIC

Applications

  • Application
    Resin screening and process optimization

Compliance Information

  • Quality Standards
    • CoA
    • RSF

Dimensions

  • Bead Size
    80 - 100 µm
  • Bed Height
    100 mm
  • Dimensions
    8 x 100 mm

General Specifications

  • Flow Rate
    200-600cm/h
  • Resin Format
    Pre-packed column

Materials of Construction

  • Base Matrix
    Rigid cellulose beads
  • Materials of Construction
    Polypropylene

Operating Conditions

  • Operating Pressure
    < 3 barg (44psi)
  • pH Stability
    1 to 14 (short term), 2 to 12 (long term)

Physicochemical Information

  • Bed Volume
    5 mL
  • Ligand
    Phenylpropylamine
  • Reference Protein
    Bovine serum albumin (BSA)
  • Typical Dynamic Binding Capacity 10% per mL
    40-60 mg/mL BSA

Product Information

  • Brand
    PPA HyperCel
  • Pack Size
    1 column per pack
  • Pack Volume
    5 mL
  • Resin Delivery Condition
    24% iPrOH/100mM sodium phosphate, pH 4.3
  • Resin Type
    Mixed-mode anion exchanger
  • What you receive
    1 column, instructions

Storage Conditions

  • Storage Conditions
    20% EtOH/1M NaCl