MEP HyperCel RoboColumn - 600 µL

Tech Support Documents
MEP HyperCel RoboColumn - 600 µL MEP HyperCel RoboColumn - 600 µL
 

MEP HyperCel provides a unique separation mechanism and selectivity for protein separations. The MEP ligand facilitates the binding of immunoglobulins as well as other target proteins at neutral pH under moderate salt concentrations. Pre-packed columns simplify resin screening and selection, and are ideal for process step optimization. The RoboColumn format is especially suited to high throughput process development (HTPD).

Bed Volume
Price (USD):
$341.00
Item No.: 
SR6MEP
Availability: 

*Custom/bulk order quotes are provided within 72 hours of request.

MEP HyperCel is a flexible mixed-mode chromatography resin designed for capture and purification of antibodies and various recombinant proteins from laboratory to manufacturing scale.

MEP HyperCel resin offers a no salt/low salt alternative to conventional HIC and can simplify processes and improve economics by removing the need for salt addition and following UF/DF steps. The MEP ligand is immunoglobulin-selective. Thus, MEP Hypercel can be effectively used for IgG capture and for enhanced DNA, HCP and aggregate removal. Elution is under mild conditions.

Features and Benefits

- Unique separation mechanism and differentiated selectivity
- Direct protein capture from a variety of feedstocks
- No salt addition required for binding means a simpler process, lower costs and reduced waste
- Stable toward harsh cleaning with NaOH


Applications

- A no-salt/low-salt alternative to Hydrophobic Interaction Chromatography (HIC)
- Monoclonal and polyclonal IgG capture and intermediate purification (aggregate, DNA and HCP removal)
- Direct capture of recombinant proteins or enzymes from feedstock without need for pH or conductivity adjustments

Applications

  • Application
    HTPD resin screening

Compliance Information

  • Quality Standards
    • CoA
    • RSF

Dimensions

  • Bead Size
    80 - 100 µm
  • Bed Height
    30 mm
  • Dimensions
    5 x 30 mm

General Specifications

  • Flow Rate
    16-1000 cm/hr
  • Resin Format
    Pre-packed column

Materials of Construction

  • Base Matrix
    Rigid cellulose beads
  • Materials of Construction
    Polypropylene

Operating Conditions

  • Operating Pressure
    < 3 barg (44psi)
  • pH Stability
    2 to 14 (short term), 2 to 12 (long term)

Physicochemical Information

  • Bed Volume
    600 µL per column
  • Ligand
    4-mercaptoethylpyridine
  • Reference Protein
    Human polyclonal IgG
  • Typical Dynamic Binding Capacity 10% per mL
    ≥ 20 mg/mL hIgG at 6' residence time

Product Information

  • Brand
    MEP HyperCel
  • Pack Size
    1 row of 8
  • Pack Volume
    600 µL
  • Resin Delivery Condition
    1M NaCl with 20% EtOH
  • Resin Type
    Mixed-mode anion exchanger
  • What you receive
    1 row of 8 columns, instructions

Storage Conditions

  • Storage Conditions
    20% EtOH/1M NaCl