INTERFERin® siRNA/miRNA Transfection

INTERFERin® provides very high silencing efficiency already at 1 nM siRNA and can be used in a wide variety of adherent and suspension cells. Easy to use thanks to its compatibility with serum and antibiotics, INTERFERin® is also perfectly suited for transfection of miRNA and other oligonucleotides like pre-miRNA, mimic miRNA, antimiR.

Amount of Reagent
Price (USD):
$72.00
Item No.: 
101000036
Availability: 

*Custom/bulk order quotes are provided within 72 hours of request.

Capabilities

Less siRNA, less off-target effects

Publications have shown that transfecting high siRNA concentrations can lead to non-specific off target effects. The reliability of the experimental data can be increased by using as little siRNA as possible. This is why INTERFERin® has been specifically designed to provide high silencing efficiency using low siRNA concentrations. INTERFERin®-mediated delivery of 1 nM of a specific siRNA shows selective and highly efficient knockdown of gene expression, while a competitor (L2K) needs at least 10 nM siRNA to reach 50% silencing efficiency.

Suitable for miRNA transfection

Transfection of microRNA (miRNA) oligonucleotides is increasingly being used to analyze biological effects of specific miRNAs on cell function. INTERFERin® is the reagent of choice for delivering miRNA, miRNA mimics or pre-miRNAs. INTERFERin® is the latest generation siRNA & miRNA transfection reagent, especially designed for high transfection efficiency in a wide variety of cells, resulting in high gene silencing or stimulation of gene expression. Indeed, some miRNA are also known to induce gene expression by association with the promoter of the gene of interest. For example, INTERFERin®-mediated delivery of miR-373 leads to a 3 fold increase in E-Cadherin expression.

Over 90 % gene silencing

For many adherent cell lines or primary cells, 1 nM siRNA is sufficient to obtain more than 90 % gene silencing. For suspension cell lines, 80 % silencing can still be reached by INTERFERin® using 5 nM siRNA.

Excellent cell viability

When it comes to cell viability, INTERFERin® outperforms other transfection reagents. 48 h after transfection with 1 nM siRNA, cells transfected with INTERFERin® appear healthy, while toxicity is clearly observed with reagent S (Fig. 3). 

Easy standard protocol

When it comes to cell viability, INTERFERin® outperforms other transfection reagents. 48 h after transfection with 1 nM siRNA, cells transfected with INTERFERin® appear healthy, while toxicity is clearly observed with reagent S.

INTERFERin® provides very high silencing efficiency already at 1 nM siRNA and can be used in a wide variety of adherent and suspension cells. Using low concentrations of siRNA avoids off-target effects, and its gentle mode of action ensures more robust data and excellent cell viability. Easy to use thanks to its compatibility with serum and antibiotics, INTERFERin® is also perfectly suited for transfection of miRNA and other oligonucleotides like pre-miRNA, mimic miRNA, and antimiR.

Key Benefits :

Efficiency: over 90% of gene silencing in cells with little as 1nM of siRNA.Excellent cell viability:  Gentle mode of action for more robust data .Versatile: Suitable for transfection miRNA and other oligonucleotides.

Applications

  • Application
    Transfection of siRNA and other oligonucleotides
  • Cell Type
    Adherent and suspension cells

Biological Information

  • Molecule delivered
    siRNA, miRNA (microRNA), and other oligonucleotides: pre-miRNA, mimic miRNA, antimiR…
  • Number of transfections
    1 ml of INTERFERin® is sufficient to perform 500-1000 transfections in 24-well plates.

General Specifications

  • Amount of Reagent
    INTERFERin® 0.1 mL
  • Reagents and Kits Type
    INTERFERin®

Storage Conditions

  • Storage Conditions
    5 °C ± 3 °C