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CIMac EDA 0.1 mL Analytical Column (1.3 µm)

Tech Support Documents
CIMac EDA 0.1 mL Analytical Column (1.3 µm)
 

This high-performance ion-exchange column is primarily intended for fast, efficient and reproducible analysis and in-process control of samples throughout different purification steps.

Channel Size
Item No.: 
110.5116-1.3
Piece
1
*Custom/bulk order quotes are provided within 72 hours of request.
CIMac EDA 0.1 mL Analytical Column (1.3 µm)
CIMac EDA 0.1 mL Analytical Column (1.3 µm)

This high-performance ion-exchange column is primarily intended for fast, efficient and reproducible analysis and in-process control of samples throughout different purification steps.

Item No.:
110.5116-1.3
Number of pieces
1

Overview

Specifications

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CIMac Analytical Columns are intended for fast and reproducible HPLC monitoring and quantitation of large proteins, plasmid DNA, viruses, virus-like particles, and phages. They were primarily designed for the in-process and final control of various samples at different stages of the purification process. They can be operated at high volumetric flow rates (from 1 to 20 column volumes/min); therefore, the information about the product quantity and purity can be obtained within a few minutes. CIMac Analytical Columns are fast and reliable PAT tools, providing:

  • Rapid, high resolution analysis, achieved in minutes
  • Flow independent separation
  • High sensitivity and reproducibility

EDA (ethylenediamine) features:

  • Weak anion exchanger
  • Working range, pH 2–9
  • Binds molecules with predominantly negative charge, repels molecules a predominantly positive charge
  • Use for capture, intermediate, or polishing steps in either bind-elute or flow-through mode
  • Sanitizable with 1 M NaOH

Applications

  • Application
    Analytical Columns

Connections (Physical)

  • Connector(s)
    10-32 UNF coned port, 0.0625" OD tubing connection

Dimensions

  • Channel Size
    1.3 µm
  • Diameter
    5.2 mm
  • Length
    4.95 mm

Features

  • Reusable
    Yes

General Specifications

  • Flow Rate
    0.2 – 3 mL/min | 1 – 15 cm/min | 2 – 30 CV/min

Materials of Construction

  • Materials of Construction
    Poly(glycidyl methacrylate -co- ethylene dimethacrylate)

Operating Conditions

  • Maximum Operating Pressure
    15 MPa | 150 bar | 2175 psi
  • Recommended Operating Temperature
    4 °C (39 °F) to 40 °C (104 °F)
  • pH Stability
    Working range 2–10, cleaning in place 1–14

Physicochemical Information

  • Bed Volume
    0.1 mL
  • Ligand
    EDA (weak anion exchanger, ethylene diamine)
  • Ligand Density
    0.7–1.1 mmol/mL wet support

Product Information

  • Brand
    CIMac™

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