CIMmic® Oligo dT18 0.1 mL Disk (C6 Linker) (2 μm channels) - Pack of 3

CIMmic^® Oligo dT is intended for capturing of ssRNA with a poly-adenylated tail, making in an ideal tool for process development screening of chromatographic conditions for your mRNA. CIMmic Oligo dT combines the accessibility of the CIMmic format with oligo-based affinity chemistry, providing a simple and flexible tool for small-scale purification and process development of mRNA.

The ligand in CIMmic^® Oligo dT18 is an 18-mer deoxythymidine chain covalently immobilised on the monolith with a carbon linker. RNA with a poly(A) tail binds to the ligand by hybridisation between adenine (A) bases on the RNA tail and deoxythymine (T) bases of the ligand. Species that do not contain a poly(A) tail do not bind to the column and are found in the unbound (flow-through) fraction.

Oligo dT (deoxythymine) features:

• Oligo dT ligands covalently bound to a solid support
• Hybridises to the poly-adenylated tail which is present on most eukaryotic mRNAs, or synthetised onto the molecule during IVT
• Primarily intended for fast and efficient capture and purification of mRNA with a poly-adenylated tail, from various sources
• Can selectively remove impurities from the sample
• Convenient approach for initial purification of mRNA

Benefits:

• Fewer unit operations: apply IVT material to monolith without buffer exchange
• Very fast processes by convective mass transport – resolution independent of flow
• High-capacity for large molecules due to fully accessible void volume in the channels
• Laminar flow minimizes shear stress resulting in high yields
• Ideal for early process development and feasibility testing
• Operable by syringe or FPLC
• Low sample and buffer consumption