CIMmultus® OH 4000 mL cGMP Compliant Monolithic Column (2 µm)

This column relies on hydrophobic interaction chromatography (HIC) to provide an excellent solution for the purification of large biomolecules, such as virus particles, VLPs and extracellular vesicles.

Item No.: 
934.8140-2

*Custom/bulk order quotes are provided within 72 hours of request.

CIMmultus® Monoliths are reusable chromatography devices for scalable high-resolution purification of complex biological samples. Inside the custom designed housing is a single piece stationary phase with homogeneous channel size and surface chemistry. This stationary phase is designed to simplify method transfer from development into production.

CIMmultus® columns can be used as single-use disposable or multi-use columns. They are available in a variety of volume ranges – from 1mL for screening and method development purposes up to 40L for industrial manufacturing.

CIMmultus® OH relies on hydrophobic interaction chromatography (HIC) to provide an excellent solution for the purification of large biomolecules, such as virus particles, VLPs and extracellular vesicles.

OH (hydroxy) features:

  • Uncharged (neutral) ligand
  • Working range, pH 2–10
  • Retains very large solutes (virus particles, VLP, vesicles) in the presence of precipitating salts or polyethylene glycol, and elutes them with high resolution in order of increasing size
  • Can be used as a pre-capture step to reduce most host cell impurities in AAV purification
  • Elution with descending salt or polyethylene glycol
  • Sanitizable with 1 M NaOH

Benefits:

  • Very fast processes by convective mass transport – resolution independent of flow
  • Ready to use columns
  • High-capacity for large molecules due to fully accessible void volume in the channels
  • Laminar flow minimizes shear stress resulting in high yields

Applications

  • Application
    Preparative Applications

Connections (Physical)

  • Connectors
    TC 1" (25 mm), 8 mm ID bore

Dimensions

  • Channel Size
    2 µm
  • Column Tube Length
    205 mm
  • Inner Diameter
    255 mm
  • Outer Diameter
    300 mm

Features

  • Reusable
    Yes

General Specifications

  • Flow Rate
    400–4000 mL/min | 13–135 cm/h | 0.1–1 CV/min

Materials of Construction

  • Materials of Construction
    Poly(glycidyl methacrylate -co- ethylene dimethacrylate)

Operating Conditions

  • Maximum Operating Pressure
    0.7 MPa | 7 bar | 100 psi
  • Recommended Operating Temperature
    4 °C (39 °F) to 40 °C (104 °F)
  • pH Stability
    Working range 3–13, cleaning in place 1–14

Physicochemical Information

  • Bed Volume
    4000 mL
  • Ligand
    OH (hydrophobic, hydroxyl)

Product Information

  • Brand
    CIMmultus®
  • Package Type
    Individually Packed