Incucyte® Neuroactive Orange Kit

A complete solution for performing kinetic, live-cell imaging of 96-well assays for sponataneous neuronal activity. Supplied as 1 vial (2 mL) Incucyte® Neuroburst OrangeLentivirus (Synapsin promoter), 1 vial (2 x 106 cells) cryopreserved Incucyte® rCortical Neurons, 1 vial (2 x 106 cells) cryopreserved Incucyte® rAstrocytes.

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The Incucyte® Neuroactive Kit contain cryopreserved vials of rat cortical neurons and astrocytes isolated from embryonic stage (E18) Sprague-Dawley rats as well as sufficient Incucyte® Neuroburst Orange Lentivirus to perform one 96-well experiment.

The Incucyte® Neuroburst Orange Lentivirus is a lentiviral based live-cell neuronal labeling reagent driven by a synapsin promoter, resulting in the long term expression of a genetically encoded orange fluorescent calcium indicator (mRuby-based) in neuronal cells. The Incucyte® Neuroburst Orange Lentivirus ensures highly efficient, yet non-disruptive labeling of primary or iPSC-derived neurons over days and weeks, and enables the kinetic quantification of neuronal activity and functional connectivity in the presence or absence of astrocytes.

The Incucyte® Neuroburst Orange Lentivirus has been validated for use with the Incucyte® S3 Live-Cell Analysis System configured with an Orange/NIR optical module and the Incucyte® SX5 Live-Cell Analysis System (Cat. No. 4816) for measurements of neuronal activity and functional connectivity with the Incucyte® Neuronal Activity Analysis Software Module (Cat. No. 9600-0032). The Neuroburst Orange Lentivirus is NOT compatible with Incucyte instruments configured with a Green/Red Optical Module, e.g. Cat. No. 4647.


  • Application
    Neuronal Activity  
  • Application Area
  • Research Area


  • Device/Instrument Compatibility
    • S3  
    • SX5  
  • Optical Module
    • Green/Orange/NIR  
    • Orange/NIR  

General Specifications

  • Color

Product Information

  • Brand
  • Pack Size


  • Volume
    2 mL  

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