Incucyte® Nuclight Green Lentivirus (EF1a, Bleo)
One vial of lentivirus reagent (>1x106 TU/mL) suitable for transducing cells with a non-perturbing, with a non-perturbing nuclear restricted green fluorescent label driven off an EF1α promoter). Stable cell lines can be created under bleomycin selection.
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Incucyte® Nuclight Green Lentivirus Reagents enable efficient, non-perturbing, nuclear labelling of living mammalian cells. They are compatible with convenient transduction protocols and enable real-time cell counting and the calculation of cell doubling times. Incucyte® Nuclight Lentivirus Reagents provide homogenous expression of a nuclear-restricted GFP (green fluorescent protein) in your choice of primary, immortalized, dividing, or non-dividing cells without altering cell function and with minimal toxicity. These reagents are ideal for generating stable cell populations or clones using puromycin selection. The Incucyte® Nuclight Lentivirus Reagents have been validated for use with the Incucyte® Live-Cell Analysis System and can be combined with the Incucyte® Caspase-3/7, Incucyte® Annexin V or Incucyte® Cytotox Dyes for multiplexed measurements of apoptosis and cytotoxicity alongside cell proliferation in a single well.
Virus Description: 3rd generation HIV-based, VSV-G pseudotyped lentiviral particles encoding a nuclear restricted GFP
Promoter: EF-1 alpha
Selectable Marker: Bleomycin
Spectral Properties: Ex (max): 483 nm; Em (max): 506 nm
Spheroid Cell Health
- Immune Cell Killing
- Nuclear Labeling
- Cell Counting
- Cell Health
- Cell Proliferation