Incucyte® Nuclight Green Lentivirus (EF1a, Bleo)

One vial of lentivirus reagent (>1x106 TU/mL) suitable for transducing cells with a non-perturbing, with a non-perturbing nuclear restricted green fluorescent label driven off an EF1α promoter). Stable cell lines can be created under bleomycin selection.

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Incucyte® Nuclight Green Lentivirus Reagents enable efficient, non-perturbing, nuclear labelling of living mammalian cells. They are compatible with convenient transduction protocols and enable real-time cell counting and the calculation of cell doubling times. Incucyte® Nuclight Lentivirus Reagents provide homogenous expression of a nuclear-restricted GFP (green fluorescent protein) in your choice of primary, immortalized, dividing, or non-dividing cells without altering cell function and with minimal toxicity. These reagents are ideal for generating stable cell populations or clones using puromycin selection. The Incucyte® Nuclight Lentivirus Reagents have been validated for use with the Incucyte® Live-Cell Analysis System and can be combined with the Incucyte® Caspase-3/7, Incucyte® Annexin V or Incucyte® Cytotox Dyes for multiplexed measurements of apoptosis and cytotoxicity alongside cell proliferation in a single well.

Virus Description: 3rd generation HIV-based, VSV-G pseudotyped lentiviral particles encoding a nuclear restricted GFP

Promoter: EF-1 alpha

Selectable Marker: Bleomycin

Spectral Properties: Ex (max): 483 nm; Em (max): 506 nm


  • Application
    • Apoptosis  
    • Cytotoxicity  
    • Spheroid Cell Health  
    • Immune Cell Killing  
  • Assay Type
    • Cell Counting  
    • Cell Health  
    • Proliferation  
    • Viability  
    • Nuclear Labeling  
  • Research Area
    • Cell Proliferation  
    • Cell Function  
    • Cell Health  


  • Device/Instrument Compatibility
    • S3  
    • SX1  
    • SX5  
  • Optical Module
    • Green/Red  
    • Green/Orange/NIR  

General Specifications

  • Color

Product Information

  • Brand
  • Pack Size


  • Volume
    0.6 mL  

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