jetOPTIMUS® DNA Transfection

Tech Support Documents
jetOPTIMUS® DNA Transfection
 

jetOPTIMUS® is an innovative cationic nanotechnology developed to improve DNA transfection efficiency in adherent cells, even in hard-to-transfect cells.  In order to work in relevant physiological conditions, transfection with jetOPTIMUS® requires a minimum DNA quantity and reagent volume which preserves cell viability and morphology.

Amount of Reagent
Price (USD):
$430.00
Item No.: 
101000025
Availability: 

*Custom/bulk order quotes are provided within 72 hours of request.

Capabilities

Superior gene expression in easy-to-hard-to-transfect cells

jjetOPTIMUS® improves DNA transfection efficiency in easy- and difficult-to-transfect cells used as in vitro cell culture models. Tested on various primary cells and cancer cell lines, jetOPTIMUS® proved its superiority by reaching higher transfection efficiencies and gene expression than main competitors.

High gene expression using low DNA amount

jetOPTIMUS® has been developed to increase transfection efficiency into a wide range of cell types, while keeping the amount of DNA as low as possible and reducing the volume of reagent required compared to main competitors. 

Excellent cell viability and morphology

Reaching high transfection efficiencies is often at the expense of cellular integrity, with adverse effects on cell cycle, metabolism and signaling pathways. At Polyplus-transfection, we make no compromise on cell integrity: jetOPTIMUS® transfection reagent is gentle on cells, preserving cell viability and morphology, which allows generation of biologically relevant data from gene expression studies.

Time-saving with optimized ready-to-use protocol

jetOPTIMUS® is a ready-to-use transfection reagent provided with its own complexation buffer (jetOPTIMUS® Buffer). The protocol is optimized for simplicity of use (all plate sizes), culture medium compatibility (antibiotics, serum) and cost-effectiveness (lowest amount of DNA and volume of reagent).

jetOPTIMUS® is a powerful transfection reagent that improves cellular uptake and endosomal escape of DNA in adherent cells resulting in higher transfection efficiency, even in hard to transfect cells. Tested on various primary cells and cancer cell lines, jetOPTIMUS® proved its superiority by reaching higher transfection efficiencies and gene expression with minimal impact on cell viability and morphology.

jetOPTIMUS® is highly efficient for DNA delivery for various applications, such as transient and stable gene expression, CRISPR genome editing using DNA approach, as well as virus production in small scale.

Key Benefits

  • Highly efficient:Maximal gene expression in easy and hard-to-transfect cells .
  • Cost-effective:Minimum reagent volume and DNA quantity required .
  • Biologically relevant:Excellent cell viability & morphology .
  • Time-saving:Transfection with an optimized ready-to-use protocol

Applications

  • Application
    • Transient and stable gene expression from plasmid DNA transfection
    • CRISPR Genome editing using DNA approach
    • Small scale virus production
  • Cell Type
    • Adherent cells and hard-to-transfect cells
    • Primary cells (epithelial, hepatocyte, endothelial, fibroblast, etc…)
    • Stem cells (embryonic, mesenchymal, induced pluripotent)
    • Cancer cell lines
    • Neurons after fibroblast

Biological Information

  • Buffer Size
    2 x 60 mL
  • Molecule delivered
    Plasmid DNA
  • Number of transfections
    1.5 ml of jetOPTIMUS® transfection reagent is sufficient to perform 3 000 transfections in 24-well plates or 750 transfections in 6-well plates following the standard protocol

General Specifications

  • Amount of Reagent
    jetOPTIMUS® 0.75 mL
  • Reagents and Kits Type
    jetOPTIMUS® - DNA transfection

Product Information

  • Provided With
    jetOPTIMUS® Buffer

Storage Conditions

  • Storage Conditions
    5 °C ± 3 °C