jetPEI® DNA Transfection

Tech Support Documents
jetPEI® DNA Transfection
 

jetPEI® transfection reagent is a linear polyethylenimine derivative, free of components of animal origin, ideal for automated or manual High Throughput Screening in adherent and suspension cells. Three protocols are available: reverse, batch and forward.

Amount of Reagent
Price (USD):
$480.00
Item No.: 
101000053
Availability: 

*Custom/bulk order quotes are provided within 72 hours of request.

Capabilities

3 protocols to suit your application

In the forward protocol, the cells are split the day before transfection and the jetPEI®/DNA complexes are added to the adherent or suspension cells.

The reverse protocol is the most appropriate when transfecting a pool of genes, such as a DNA library. In this protocol, the jetPEI®/DNA complexes are prepared or deposited in the wells prior to addition of the cells. Complexes are stable for up to 4 hours

The batch protocol has been developed to prepare a homogeneous pool of transfected cells. For this purpose, the cells are transfected just after trypsinization, while still in suspension. This protocol is preferred for drug screening applications and allows rapid processing, one day faster than the forward protocol.

Robust transfection complexes for reliable HTS

Complexes formed with the water-soluble polymer jetPEI® and DNA allow efficient transfection for up to 4 hours, in contrast to lipid-based reagents and calcium phosphate. Thus they allow plenty of time to dispense the complexes into the plates.

Efficient in a wide range of cell types

jetPEI® successfully delivers genes to various adherent and non-adherent cell lines, as well as primary cells. Over 550 publications using jetPEI® can be found . In addition, our online Database gives specific transfection conditions for over 400 cell lines and primary cells.

Superior transfection results

jetPEI® was compared to several other popular transfection reagents. jetPEI® was found to offer the best performance: high efficiency and low variability.

High-Throughput Screening (HTS) is widely used in pharmaceutical industry and basic & translational research to study biological processes. HTS bioassays are almost exclusively conducted in the microtiter plate formats (96, 384 or 1536 wells), which makes the use of a highly reproducible transfection reagent indispensable.

Key Benefits:

Versatile:Well-suited for adherent and suspension cells.Efficient:Fast methods to transfect cells for HTS.Robust:Exceptionally reproducible results.Flexible:Reverse, batch & forward protocols available.

Applications

  • Application
    • Plasmid transfection
    • High Throughput Screening (HTS)
  • Cell Type
    Adherent and suspension cells

Biological Information

  • Amount of NaCl
    50 mL
  • Molecule delivered
    DNA
  • Number of transfections
    1 ml of jetPEI® is sufficient to perform up to 2000 transfections in 96-well plates.

General Specifications

  • Amount of Reagent
    jetPEI® 1 mL
  • Reagents and Kits Type
    jetPEI®

Product Information

  • Provided With
    Provided with 150 mM NaCl solution for complex formation

Storage Conditions

  • Storage Conditions
    5 °C ± 3 °C