MEP HyperCel PRC Column - 5 mL
MEP HyperCel provides a unique separation mechanism and selectivity for protein separations. The MEP ligand facilitates the binding of immunoglobulins as well as other target proteins at neutral pH under moderate salt concentrations. Pre-packed columns simplify resin screening and selection, and are ideal for process step optimization. The RoboColumn format is especially suited to high throughput process development (HTPD).
- Price (USD):
- $243.00
- Item No.:
- PRC08X100MEPHCEL
*Custom/bulk order quotes are provided within 72 hours of request.
Choose your MEP HyperCel
MEP HyperCel is a flexible mixed-mode chromatography resin designed for capture and purification of antibodies and various recombinant proteins from laboratory to manufacturing scale.
MEP HyperCel resin offers a no salt/low salt alternative to conventional HIC and can simplify processes and improve economics by removing the need for salt addition and following UF/DF steps. The MEP ligand is immunoglobulin-selective. Thus, MEP Hypercel can be effectively used for IgG capture and for enhanced DNA, HCP and aggregate removal. Elution is under mild conditions.
Features and Benefits
- Unique separation mechanism and differentiated selectivity
- Direct protein capture from a variety of feedstocks
- No salt addition required for binding means a simpler process, lower costs and reduced waste
- Stable toward harsh cleaning with NaOH
Applications
- A no-salt/low-salt alternative to Hydrophobic Interaction Chromatography (HIC)
- Monoclonal and polyclonal IgG capture and intermediate purification (aggregate, DNA and HCP removal)
- Direct capture of recombinant proteins or enzymes from feedstock without need for pH or conductivity adjustments
Application Notes
Datasheet
User Manual
Applications
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ApplicationResin screening and process optimization
Compliance Information
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Quality Standards
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CoA
- RSF
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CoA
Dimensions
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Bead Size80 - 100 µm
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Bed Height100 mm
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Dimensions8 x 100 mm
General Specifications
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Flow Rate200-600cm/h
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Resin FormatPre-packed column
Materials of Construction
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Base MatrixRigid cellulose beads
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Materials of ConstructionPolypropylene
Operating Conditions
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Operating Pressure< 3 barg (44psi)
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pH Stability2 to 14 (short term), 2 to 12 (long term)
Physicochemical Information
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Bed Volume5 mL
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Ligand4-mercaptoethylpyridine
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Reference ProteinHuman polyclonal IgG
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Typical Dynamic Binding Capacity 10% per mL≥ 20 mg/mL hIgG at 6' residence time
Product Information
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BrandMEP HyperCel
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Pack Size1 column per pack
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Pack Volume5 mL
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Resin Delivery Condition1M NaCl with 20% EtOH
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Resin TypeMixed-mode anion exchanger
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What you receive1 column, instructions
Storage Conditions
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Storage Conditions20% EtOH/1M NaCl