Sartobind® Lab IDA Metal Affinity Membrane Adsorbers

Top Features of Sartobind® Lab IDA Metal Affinity Membrane Adsorbers

  • Achieve optimum his-tagged protein purity and yield, using the metal ion of your choice
  • Higher flow rates compared with metal affinity resins
  • Operate by syringe, peristaltic pump or FPLC system

Popular Applications for Sartobind® Lab IDA Metal Affinity Membrane Adsorbers

  • His-tagged protein purification
  • Screening
  • Optimization

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*Custom/bulk order quotes are provided within 24 hours of request.

Based on the Patented Sartobind® Platform

Sartobind® Lab membrane adsorbers for immobilized metal affinity chromatography (IMAC) are ready-to-use units in a syringe filter format. They are based on the patented macroporous Sartobind® membranes, with iminodiacetic acid (IDA) covalently bound to the entire internal surface of the pores. This results in a chromatography matrix with superior flow rates and high binding capacity. The IDA ligand enables coupling of the metal ion of your choice to the membrane adsorber, ensuring simple process optimization for maximum purity and yield of each his-tagged protein.

Ultimate Versatility

Sartobind® Lab IDA units are available with a 2.1 mL bed volume, providing binding capacities up to 7.5 mg his-tagged protein per unit. Equipped with standard Luer lock connectors, Sartobind® Lab units enable simple, equipment-free handling by syringe, or can be easily connected to FPLC systems for automated purifications. The units are compatible with most common chromatography buffers and can be regenerated for multiple purification cycles, ensuring high-cost efficiency.


  • Application
    • Screening  
    • Optimization  
    • His-tagged Protein Purification  
  • Intended Use
    Research Use Only (RUO)  


  • Bed Height
    4 mm  
  • Membrane Area
    75 cm²  


  • Reusable

Materials of Construction

  • Housing Material
    Polypropylene (PP)  
  • Membrane Material
    Stabilized reinforced cellulose  
  • Reinforcement Material

Operating Conditions

  • Maximum Operating Pressure
    0.6 MPa  
  • Recommended Flow Rate
    10-63 mL/min (5-30 MV/min)  
  • pH Stability
    pH 1 – 12 (short term), pH 3 – 9 (long term)  

Physicochemical Information

  • Bed Volume
    2.1 mL  
  • Chemical Compatibility
    Stable in common chromatography buffers, avoid peroxide and other oxidizing or reactive reagents  
  • Ligand
    Iminodiacetic acid (IDA)  
  • Ligand Density
    5 µeq/cm²  
  • Pore Size
    3 - 5 µm  
  • Recommended Chelating Ions
    Ni2+, Co2+, Cu2+, or Zn2+  
  • Reference Protein
    Polyhistidine tagged protein  
  • Typical Dynamic Binding Capacity 10% per unit
    7.5 mg  
  • Void Volume
    1.3 mL  

Product Information

  • Brand
    Sartobind® Lab  
  • Membrane Type
    Metal chelate  
  • Pack Size
  • What you receive
    • 2 units  
    • 1 operating instruction sheet  
  • Sartobind® Adapter UNF 10-32 female to Luer male

    Adapter UNF 10-32 female to Luer male for Sartobind®Material: natural PEEK

  • Luer Female to UNF 10-32 Female Adapters, 2 pc


    Luer female/UNF 10-32 female adapters, for Sartobind® Lab units.