jetOPTIMUS®

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jetOPTIMUS®
 

jetOPTIMUS® is an innovative cationic nanotechnology developed to improve DNA transfection efficiency in adherent cells, even in hard-to-transfect cells.  In order to work in relevant physiological conditions, transfection with jetOPTIMUS® requires a minimum DNA quantity and reagent volume which preserves cell viability and morphology.

Amount of Reagent
Item No.: 
101000006

*Custom/bulk order quotes are provided within 72 hours of request.

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jetOPTIMUS® is a powerful transfection reagent that improves cellular uptake and endosomal escape of DNA in adherent cells resulting in higher transfection efficiency, even in hard to transfect cells. Tested on various primary cells and cancer cell lines, jetOPTIMUS® proved its superiority by reaching higher transfection efficiencies and gene expression with minimal impact on cell viability and morphology.

jetOPTIMUS® is highly efficient for DNA delivery for various applications, such as transient and stable gene expression, CRISPR genome editing using DNA approach, as well as virus production in small scale.

Key Benefits

  • Highly efficient:Maximal gene expression in easy and hard-to-transfect cells .
  • Cost-effective:Minimum reagent volume and DNA quantity required .
  • Biologically relevant:Excellent cell viability & morphology .
  • Time-saving:Transfection with an optimized ready-to-use protocol

Applications

  • Application
    • Transient and stable gene expression from plasmid DNA transfection  
    • CRISPR Genome editing using DNA approach  
    • Small scale virus production  
  • Cell Type
    • Adherent cells and hard-to-transfect cells  
    • Primary cells (epithelial, hepatocyte, endothelial, fibroblast, etc…)  
    • Stem cells (embryonic, mesenchymal, induced pluripotent)  
    • Cancer cell lines  
    • Neurons after fibroblast  

Biological Information

  • Molecule delivered
    Plasmid DNA  
  • Number of transfections
    1.5 ml of jetOPTIMUS® transfection reagent is sufficient to perform 3 000 transfections in 24-well plates or 750 transfections in 6-well plates following the standard protocol  

General Specifications

  • Amount of Reagent
    jetOPTIMUS® 1.5 mL  
  • Reagents and Kits Type
    jetOPTIMUS® - DNA transfection  

Product Information

  • Provided With
    jetOPTIMUS® Buffer  

Storage Conditions

  • Storage Conditions
    5 °C ± 3 °C