Sartobind® Lab IDA Metal Affinity Membrane Adsorbers

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Sartobind® Lab IDA Metal Affinity Membrane Adsorbers
 

Top Features of Sartobind® Lab IDA Metal Affinity Membrane Adsorbers

  • Achieve optimum his-tagged protein purity and yield, using the metal ion of your choice
  • Higher flow rates compared with metal affinity resins
  • Operate by syringe, peristaltic pump or FPLC system

Popular Applications for Sartobind® Lab IDA Metal Affinity Membrane Adsorbers

  • His-tagged protein purification
  • Screening
  • Optimization

Bed Volume
Price (USD):
$284.00
Item No.: 
93IDA-42DB-12--V
Availability: 

*Custom/bulk order quotes are provided within 24 hours of request.

Choose your Sartobind® Lab Units (not scalable)

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Based on the Patented Sartobind® Platform

Sartobind® Lab membrane adsorbers for immobilized metal affinity chromatography (IMAC) are ready-to-use units in a syringe filter format. They are based on the patented macroporous Sartobind® membranes, with iminodiacetic acid (IDA) covalently bound to the entire internal surface of the pores. This results in a chromatography matrix with superior flow rates and high binding capacity. The IDA ligand enables coupling of the metal ion of your choice to the membrane adsorber, ensuring simple process optimization for maximum purity and yield of each his-tagged protein.

Ultimate Versatility

Sartobind® Lab IDA units are available with a 2.1 mL bed volume, providing binding capacities up to 7.5 mg his-tagged protein per unit. Equipped with standard Luer lock connectors, Sartobind® Lab units enable simple, equipment-free handling by syringe, or can be easily connected to FPLC systems for automated purifications. The units are compatible with most common chromatography buffers and can be regenerated for multiple purification cycles, ensuring high-cost efficiency.

Applications

  • Application
    • 8799048172128  
    • 8801204896352  
    • 8801204929120  
  • Intended Use
    8804055712352  

Dimensions

  • Bed Height
    4 mm  
  • Membrane Area
    75 cm²  

Features

  • Reusable
    8805465031264  

Materials of Construction

  • Housing Material
    8804580328032  
  • Membrane Material
    8799043289696  
  • Reinforcement Material
    8799038898784  

Operating Conditions

  • Maximum Operating Pressure
    0.6 MPa  
  • Recommended Flow Rate
    10-63 mL/min (5-30 MV/min)  
  • pH Stability
    pH 1 – 12 (short term), pH 3 – 9 (long term)  

Physicochemical Information

  • Bed Volume
    2.1 mL  
  • Chemical Compatibility
    8801204863584  
  • Ligand
    8805269602912  
  • Ligand Density
    5 µeq/cm²  
  • Pore Size
    3 - 5 µm  
  • Recommended Chelating Ions
    Ni2+, Co2+, Cu2+, or Zn2+  
  • Reference Protein
    8799081005664  
  • Typical Dynamic Binding Capacity 10% per unit
    7.5 mg  
  • Void Volume
    1.3 mL  

Product Information

  • Brand
    8801139327584  
  • Membrane Type
    8799042339424  
  • Pack Size
    2  
  • What you receive
    • 2 units  
    • 1 operating instruction sheet  
  • Sartobind® Adapter UNF 10-32 female to Luer male
    Sartobind® Adapter UNF 10-32 female to Luer male
    Item no.
    1ZA---0004

    Adapter UNF 10-32 female to Luer male for Sartobind®Material: natural PEEK

  • Luer Female to UNF 10-32 Female Adapters, 2 pc
    Luer Female to UNF 10-32 Female Adapters, 2 pc
    Item no.
    1ZA---0005

    $57.20

    Luer female/UNF 10-32 female adapters, for Sartobind® Lab units.

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