CIMac™ EDA 0.1 mL Analytical Column (2 µm)
CIMac™ Analytical Columns combine all elaborate characteristics of CIM® monoliths at analytical scale and are your perfect tool for analysis and control of manufacturing processes, ideal for process analytical technology (PAT).
Designed for fast and reproducible HPLC monitoring and quantitation of proteins, viruses, pDNA, and other large biomolecules, they allow you to achieve rapid, high-resolution and flow-independent separations in a matter of minutes.
- Item No.:
- 110.5116-2
- Piece
- 1
*Custom/bulk order quotes are provided within 72 hours of request.
Choose your CIMac™
The CIMac™ EDA 0.1 mL Analytical Columns are weak anion-exchange monolithic columns, that bind biomolecules that are predominantly negatively charged over the pH working range of 3-9. In contrast to DEAE or QA monoliths it contains free primary amino groups, resulting in a promotion of not purely ion-exchange interaction.
EDA (ethylenediamine) features:
- Weak anion exchanger
- Working range, pH 3–9
- Binds molecules with predominantly negative charge, repels molecules a predominantly positive charge
- Use for capture, intermediate, or polishing steps in either bind-elute or flow-through mode
- High capacity for plasmid DNA
- Sanitizable with 1 M NaOH
Applications
-
ApplicationAnalytical Columns
Connections (Physical)
-
Connectors10-32 UNF coned port, 0.0625" OD tubing connection
Dimensions
-
Channel Size2 µm
-
Diameter5.2 mm
-
Length4.95 mm
Features
-
ReusableYes
General Specifications
-
Flow Rate0.2 – 3 mL/min | 1 – 15 cm/min | 2 – 30 CV/min
Materials of Construction
-
Materials of ConstructionPoly(glycidyl methacrylate -co- ethylene dimethacrylate)
Operating Conditions
-
Maximum Operating Pressure16 MPa | 150 bar | 2175 psi
-
Recommended Operating Temperature5 °C (39 °F) to 40 °C (104 °F)
-
pH StabilityWorking range 2–10, cleaning in place 1–15
Physicochemical Information
-
Bed Volume0.1 mL
-
LigandEDA (weak anion exchanger, ethylene diamine)
Product Information
-
BrandCIMac™
Biocompatible analytical chromatography system with quaternary buffer setup.
PATfix® analytical quaternary system is an at-line tool, to help process developers of novel gene therapies get immediate feedback on their current process state. This enables faster iteration and process optimization towards better purities and higher recoveries. The Sartorius analytical HPLC system provides modality-appropriate analysis and in-process control for gene therapy and vaccine products. Designed to take full advantage of the capabilities of CIMac™ analytical monolithic columns, the PATfix® HPLC delivers fast, high-resolution runs. Operation with multiple detector types provides content- rich analyses even with complex samples. User friendly PATfix® software makes for easy data processing, visualization and sharing.