CIMac™ HDZ 0.1 mL Analytical Column (1.3 µm)
Hydrazide activated monolithic columns are used to immobilise glycoproteins through oxidised sugar groups and other carbonyl containing molecules.
Antibodies, in particular immunoglobulins G (IgG) containing abundant glycosylation, can be covalently bound to the matrix leaving unobstructed antigen binding sites.
- Item No.:
- 110.8002-1.3
*Custom/bulk order quotes are provided within 72 hours of request.
Choose your CIMac™
CIMac™ Analytical Columns are short-bed high-performance monolithic columns offering all the advantages of CIM® monolithic technology. Their small volume and short column length allow the operation at high volumetric flow rates, enabling them to receive the information about the product quantity and purity in just a few minutes. Hence, the CIMac™ Analytical Columns can be effectively used for the in-process and final control of various samples from different purification process steps
.The CIMac HDZ–0.1 Analytical Columns are used to immobilise glycoproteins through oxidised sugar groups and other carbonyl containing molecules. Antibodies, in particular immunoglobulins G (IgG) containing abundant glycosylation, can be covalently bound to the matrix leaving unobstructed antigen binding sites. The resulting affinity columns provide high purification efficiency and no leaching of the immobilised ligand.
HDZ (hydrazide activated) features:
- For easy preparation of efficient immunoaffinity columns.
- Forms a covalent hydrazide linkage with carbohydrates.
- Especially suited for site-directed immobilization of antibodies and other glycoproteins.
Applications
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ApplicationAnalytical Columns
Connections (Physical)
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Connectors10-32 UNF coned port, 0.0625" OD tubing connection
Dimensions
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Channel Size1.3 µm
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Diameter5.2 mm
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Length4.95 mm
Features
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ReusableYes
General Specifications
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Flow Rate0.2 – 3 mL/min | 1 – 15 cm/min | 2 – 30 CV/min
Materials of Construction
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Materials of ConstructionPoly(glycidyl methacrylate -co- ethylene dimethacrylate)
Operating Conditions
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Maximum Operating Pressure15 MPa | 150 bar | 2175 psi
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Recommended Operating Temperature8 °C (46 °F) to 35 °C (95 °F)
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pH StabilityWorking range 4–10
Physicochemical Information
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Bed Volume0,1 mL
Product Information
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BrandCIMac™
Biocompatible analytical chromatography system with quaternary buffer setup.
PATfix® analytical quaternary system is an at-line tool, to help process developers of novel gene therapies get immediate feedback on their current process state. This enables faster iteration and process optimization towards better purities and higher recoveries. The Sartorius analytical HPLC system provides modality-appropriate analysis and in-process control for gene therapy and vaccine products. Designed to take full advantage of the capabilities of CIMac™ analytical monolithic columns, the PATfix® HPLC delivers fast, high-resolution runs. Operation with multiple detector types provides content- rich analyses even with complex samples. User friendly PATfix® software makes for easy data processing, visualization and sharing.