Incucyte® Neurolight Lentivirus (Synapsin)

The Incucyte^® Neurolight Lentivirus Reagents are third generation HIV-based, VSV-G pseudotyped lentiviral particles to enable highly-efficient, yet non-disruptive labeling of primary or iPSC-derived neurons. Once integrated into cells of interest, a neuron-specific (synapsin) promoter drives stable, long-term expression of a red or orange fluorescent protein (mKate2 or TagRFP, respectively) in neuronal cell bodies and neurites. This enables the kinetic quantification of neurite length and branching over a period of several weeks, even in the presence of astrocytes and other non-neuronal cell types such as microglia. The Incucyte^® Neurolight Red and Orange Lentivirus Reagents have been validated for use with the Incucyte^® Live-Cell Analysis Systems for measurements of neurite outgrowth, maturation, and the disruption of neurite networks.