MEP HyperCel

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MEP HyperCel MEP HyperCel MEP HyperCel
 

MEP HyperCel provides a unique separation mechanism and selectivity for protein separations, with ligand-based immunoglobulin selectivity optimal for monoclonal antibody capture and intermediate purification. The MEP ligand facilitates the binding of immunoglobulins as well as other target proteins at neutral pH under moderate salt concentrations.

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MEP HyperCel
MEP HyperCel

MEP HyperCel provides a unique separation mechanism and selectivity for protein separations, with ligand-based immunoglobulin selectivity optimal for monoclonal antibody capture and intermediate purification. The MEP ligand facilitates the binding of immunoglobulins as well as other target proteins at neutral pH under moderate salt concentrations.

Item No.:
M_MEP_HyperCel

Overview

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MEP HyperCel is a flexible mixed mode chromatography resin designed for capture and purification of antibodies and various recombinant proteins from laboratory to manufacturing scale. MEP HyperCel resin offers a no salt/low salt alternative to conventional HIC and can simplify processes and improve economics by removing the need for salt addition and following UF/DF steps. The MEP ligand is immunoglobulin-selective. Thus, MEP Hypercel can be effectively used for IgG capture and for enhanced DNA, HCP and aggregate removal. Elution is under mild conditions.

Features and Benefits

  • Unique separation mechanism and differentiated selectivity
  • Direct protein capture from a variety of feedstocks
  • No salt addition required for binding means a simpler process, lower costs and reduced waste
  • Stable toward harsh cleaning with NaOH

Applications

  • A no-salt/low-salt alternative to Hydrophobic Interaction Chromatography (HIC)
  • Monoclonal and polyclonal IgG capture and intermediate purification (aggregate, DNA and HCP removal)
  • Direct capture of recombinant proteins or enzymes from feedstock without need for pH or conductivity adjustments

Compliance Information

  • Quality Standards
    • CoA
    • RSF (upon request)

Dimensions

  • Bead Size
    80 - 100 µm

Materials of Construction

  • Base Matrix
    Rigid cellulose beads

Operating Conditions

  • Operating Pressure
    < 3 barg (44psi)
  • pH Stability
    2 to 14 (short term), 2 to 12 (long term)

Physicochemical Information

  • Ligand
    4-mercaptoethylpyridine
  • Reference Protein
    Human polyclonal IgG
  • Typical Dynamic Binding Capacity 10% per mL
    ≥ 20 mg/mL hIgG at 6' residence time

Product Information

  • Brand
    MEP HyperCel
  • Resin Delivery Condition
    1M NaCl with 20% EtOH
  • Resin Type
    Mixed Mode anion exchanger

Storage Conditions

  • Storage Conditions
    20% EtOH/1M NaCl