CIMac™ C4 HLD 0.1 mL Analytical Column (2 µm)

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CIMac™ C4 HLD 0.1 mL Analytical Column (2 µm)
 

CIMac™ Analytical Columns combine all elaborate characteristics of CIM® monoliths at analytical scale and are your perfect tool for analysis and control of manufacturing processes, ideal for process analytical technology (PAT).

Designed for fast and reproducible HPLC monitoring and quantitation of proteins, viruses, pDNA, and other large biomolecules, they allow you to achieve rapid, high-resolution and flow-independent separations in a matter of minutes.

Item No.: 
110.8136-2

*Custom/bulk order quotes are provided within 72 hours of request.

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CIMac™ Analytical Columns are short-bed high-performance monolithic columns offering all the advantages of CIM® monolithic technology. Their small volume and short length enable high volumetric flow rates, allowing for the rapid acquisition of quantity and purity information within minutes.Hence, the CIMac™ Analytical Columns are ideal for the in-process and final control of various samples from different purification process steps.

Features:

  • Volume of 0.1 mL (0.3 mL for CIMac™ pDNA)
  • Flow rates from 1 to 30 column volumes/minute (varies by type)
  • Application-optimized channel widths
  • Available in a wide variety of chemistries

Benefits:

  • Analytical HPLC columns for nucleic acids, viral vectors, viruses, and other biomolecules
  • In-process analysis and quantitation, and final product
  • At-line monitoring and control of manufacturing processes (PAT)
  • Rapid, high-resolution analysis achieved in minutes
  • High sensitivity, accuracy and reproducibility
  • No sample carryover

Applications

  • Application
    Analytical Columns

Connections (Physical)

  • Connectors
    10-32 UNF coned port, 0.0625" OD tubing connection

Dimensions

  • Channel Size
    2 µm
  • Diameter
    5.2 mm
  • Length
    4.95 mm

Features

  • Reusable
    Yes

General Specifications

  • Flow Rate
    0.2 – 3 mL/min | 1 – 15 cm/min | 2 – 30 CV/min

Materials of Construction

  • Materials of Construction
    Poly(butyl methacrylate -co- ethylene dimethacrylate)

Operating Conditions

  • Maximum Operating Pressure
    15 MPa | 150 bar | 2175 psi
  • Recommended Operating Temperature
    2 °C (36 °F) to 25 °C (77 °F)
  • pH Stability
    Working range 3–13, cleaning in place 1–14

Physicochemical Information

  • Bed Volume
    0,1 mL
  • Ligand
    C4 HLD (hydrophobic, butyl – high ligand density)
  • Typical Dynamic Binding Capacity 10% per mL
    2.5–3.5 mg pEGFP/mL wet support, pEGFP (4.7 kbp) 0.04 mg/mL, 50 mM TRIS, 10 mMEDTA, 3 M (NH₄)₂SO₄, pH 7.2

Product Information

  • Brand
    CIMac™