Incucyte® Exofluor Green EV Labeling Kit
One kit containing one (1) vial of lyophilized Incucyte® Exofluor Green EV Labeling Dye, ten (10) Vivaspin® 2 concentrators, and ten (10) Minisart® PES 0.22 µm filters - suitable for ten labeling reactions.
Top Features of Incucyte® Exofluor Green EV Labeling Kit
- Label EVs to observe and evaluate kinetic changes in intracellular EV uptake
- Preserve precious EVs and biological functionality with simple assay protocol
- Monitor EV uptake with fluorescent labeling dye compatible with EVs extracted from different methods
Top Applications for Incucyte® Exofluor Green EV Labeling Kit
- Cancer research
- Therapeutics and drug discovery
- Biomarker identification
Please note that all sales are final and that this product is not returnable, with the exception of warranty issues covered under Sartorius standard terms and conditions. If you have any issues with this product, please contact your Sartorius sales or service representative for assistance.
- Price (USD):
- $680.00
- Item No.:
- BA-04877
- Availability:
- pcs
*Custom/bulk order quotes are provided within 24 hours of request.
Choose your Incucyte® Reagents and Kits
Extracellular vesicles (EVs) are heterogeneous, lipid-bound particles (50 – 1000 nm) naturally secreted by cells into the extracellular space. Exosomes, a subclass of EVs, are defined by their size (40 to 160 nm) and biogenesis (endosomal origin). EVs, which transport biomolecules like proteins, lipids and nucleic acids, are of interest to researchers for diagnostic and therapeutic purposes. The Incucyte® Exofluor Green EV Labeling Kit allows for real-time evaluation of cellular EV uptake and is validated for use with the Incucyte® Live-Cell Analysis System. The Incucyte® Exofluor Green EV Labeling Dye labels the plasma membrane of small EVs by covalently binding to proteins and amino acids. The irreversible binding to the EVs, along with the removal of free dye, reduces the risk of non-specific dye attachment to other plasma membranes.
The Incucyte® Exofluor Green EV Labeling Kit is designed to label EVs, such as exosomes, for investigation in live-cell assays using the Incucyte® Live-Cell Analysis System. The kit includes Incucyte® Exofluor Green EV Labeling Dye for labeling EVs, Vivaspin®2 concentrators for removing free dye, and Minisart® PES 0.22 µm filters for sterilization and removal of unbound aggregate dye. After processing, labeled EVs are added to cells, and uptake is measured by quantifying green fluorescence. The kit is compatible with EVs isolated using various methods and contains components for 10 labeling reactions. It is intended for use in the Incucyte® SX5, S3, or SX1 Live-Cell Analysis Systems that are configured with either a Green/Red or a Green/Orange/NIR Optical Module.
Recommended Use
We recommend that the Incucyte® Exofluor Green EV Labeling Dye is prepared at a 5 mM stock concentration using anhydrous DMSO (not supplied). The dye should then be diluted 1:10 with Phosphate Buffer Saline (Ca²+/Mg²+ free) and used at a final concentration of 80 μM in a 30-minute labeling reaction. The volume of EVs needed for each reaction depends on the number of assay wells and the concentration of EVs per well. The recommended range of EVs is 1 – 4 μg of protein/well or 5 x 10⁷ – 1 x 10⁹ particles/well – these ranges have been validated via testing of a wide range of exosome extraction methods, however some optimization may be required. Vivaspin® 2 columns are used to remove unlabeled dye from the labeled EVs, with a maximum load of 40 μg protein or 100 μL labeled EV sample. Exceeding these limits may cause membrane clogging or increased background fluorescence. After removing the free dye, a Minisart® PES 0.22 µm syringe filter is used for sterilization and removal of unbound aggregates.
Applications
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ApplicationEV/Exosome Research
-
Assay TypeEV Labeling
Features
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Device/Instrument Compatibility
-
S3
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SX1
- SX5
-
S3
-
Optical Module
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Green/Red
- Green/Orange/NIR
-
Green/Red
General Specifications
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ColorGreen
Product Information
-
BrandIncucyte®
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