The mFix4 mRNA standard is a 3969 nt long mRNA molecule with a poly-adenylated tail of 95 nt. The molecule is produced by IVT reaction with a DNA template retrieved from recombinant microbial expression (E. coli). The standard contains 95 ± 2 % polyadenylated mRNA and is primarily used for System Suitability Testing.
Content:
- 1 x vial of 300 µL sample (150 µg of mRNA standard) (Cat. No. BIA-mFix4.1.1) - Packing buffer: 1 mM Sodium Citrate, pH 6.4
Equipment required:
- PATfix®-model-mRNA (analytical system with quaternary pump) (Cat. No. PAT0050)
Consumables that are not included:
- CIMac™ Oligo dT column (Cat. No. 110.1219-2),
- CIMac PrimaS™ column (Cat. No. 110.5118-2) (OR??)
- CIMac™ SDVB column (Cat. No. BIA-110.9001-2), vials for the PATfix® system
- 0.22 µm PES filter
Standard storage:
- Long term storage: -80°C
- Short term storage: mFix4 can be stored up to 6 months at -20°C
- Standard shipment at 2-8°C. Standard is stable up to two weeks at this temperature. Avoid multiple freeze-thaw cycles.
