A well established transfection medium with HEK293 Cell Lines in suspension mode !
HEK TF is achemically defined, animal-component-free, serum free and hydrolysate-free medium It was developed for cultivation of HEK293 and other mammalian cell lines, with a special focus on transfection applications and virus production (e.g. AAV production and lentivirus production) in suspension , making it the perfect choice for your gene therapy applications. The medium is especially suited for transient transfection with e.g. polycationic transfection reagents such as polyethylenimine (PEI). It contains a medium level of nutrients and growth factors. It can be used in research or in manufacturing applications and for all steps such as thawing, expanding, pre-complexing DNA, transfecting and producing steps can be performed in the same medium, regardless of the production scale.