CIM® OH 1 mL Monolithic 24-well Plate (6 μm channels)

We combined our unique CIM^® monolith technology with a standard multi-well plate design. Whether your goal is to screen multiple chromatographic conditions at once or high throughput purification of your target molecule, our CIM^® plates offer an answer for you.

Each well in our monolithic plates is prefilled with a defined amount of monolith to enable uniform high flow rates across the plate. The properties of the monoliths in the wells are comparable to our preparative chromatographic monolithic columns. This makes plates an ideal tool for screening different parts of your chromatographic process.

CIM^® plates can be disposable or multi-use and are made from medical grade polypropylene (PP), a material that prevents target molecules from binding to the plastic. They are manufactured according to ANSI standards and are automation compatible. Nevertheless, they can also be operated manually with a vacuum, centrifuge, or positive pressure.

OH (hydroxy) features:

• Uncharged (neutral) ligand
• Working range, pH 2–10
• Retains very large solutes (virus particles, VLP, vesicles) in the presence of precipitating salts or polyethylene glycol, and elutes them with high resolution in order of increasing size
• Can be used as a pre-capture step to reduce most host cell impurities in AAV purification
• Elution with descending salt or polyethylene glycol
• Sanitizable with 1 M NaOH

Application

• Screening of chromatographic conditions (binding, washing, elution conditions)
• High throughput purification of large biomolecules (virus particles, VLP, extracellular vesicles)

Benefits

• Simplicity of operation: No shaking and incubation required.
• Easy transfer of the results to a larger scale: Monoliths in well-plates have the same properties as larger preparative monoliths (CIMmultus^®), allowing simultaneous screening of multiple conditions and easy scale-up.
• Time & money savings: Increased productivity by quick processing time and reduced sample consumption.
• Monolith technology benefits: Up to 10-times greater capacities compared to other resin-based columns for large biomolecules due to high surface availability of binding sites. Low shears allow 3-times higher yields for particles and large biomolecules compared to other purification resins.