CIMac™ DEAE 0.1 mL Analytical Column (1.3 µm)
Choose your CIMac™
CIMac™ Analytical Columns are short-bed high-performance monolithic columns offering all the advantages of CIM® monolithic technology. Their small volume and short column length allow the operation at high volumetric flow rates, enabling them to receive the information about the product quantity and purity in just a few minutes. Hence, the CIMac™ Analytical Columns can be effectively used for the in-process and final control of various samples from different purification process steps.
The CIMac™ DEAE-0.1 Analytical Columns are weak anion-exchange monolithic columns, that bind biomolecules that are predominantly negatively charged over the pH working range of 3-9.
DEAE (diethylamine) features:
- Weak anion exchanger
- Working range, pH 2–9
- Binds molecules with predominantly negative charge, repels molecules a predominantly positive charge
- Use for capture, intermediate, or polishing steps in either bind-elute or flow-through mode
- High capacity for plasmid DNA
- Sanitizable with 1 M NaOH
Applications
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ApplicationAnalytical Columns
Connections (Physical)
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Connector(s)10-32 UNF coned port, 0.0625" OD tubing connection
Dimensions
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Channel Size1.3 µm
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Diameter5.2 mm
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Length4.95 mm
Features
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ReusableYes
General Specifications
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Flow Rate0.2 – 3 mL/min | 1 – 15 cm/min | 2 – 30 CV/min
Materials of Construction
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Materials of ConstructionPoly(glycidyl methacrylate -co- ethylene dimethacrylate)
Operating Conditions
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Maximum Operating Pressure15 MPa | 150 bar | 2175 psi
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Recommended Operating Temperature4 °C (39 °F) to 40 °C (104 °F)
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pH StabilityWorking range 2–10, cleaning in place 1–14
Physicochemical Information
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Bed Volume0.1 mL
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LigandDEAE (weak anion exchanger, diethylamino)
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Typical Dynamic Binding Capacity 10% per mL25–31 mg BSA/mL wet support, BSA, 0.5 mg/mL, 20 mM Tris-HCI buffer, pH 7.4, flow rate 1 mL/min
Product Information
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BrandCIMac™