CIMmultus® HiP2 Plasmid Process Pack® 800-800 mL

CIMmultus^® HiP² Plasmid Process Pack^® is a ready-to-use, yet optionally adjustable pDNA purification pack, intended to help scientists develop their own pDNA purification process and transfer it to production.Detailed instructions of the experiments are included in the pack, thus helping you to save time and money on all scales. The process is extremely flexible, as it also allows choosing only parts of the recommended protocol that best fit your needs.

CIMmultus^® HiP² Plasmid Process Pack^® contains one weak anion-exchange (DEAE) and one hydrophobic (C4 HLD) monolithic column, along with a protocol describing the purification procedure of your particular plasmid.

Contaminants are removed without the use of enzymatic treatment. Potassium acetate and calcium chloride precipitation, alongside two separate chromatography steps, are used for removal of RNA, endotoxin, genomic DNA, protein and other contaminants.

• The first chromatography step (DEAE) separates contaminant RNA and proteins from the plasmid DNA.
• The polishing step (C4 HLD) eliminates remains of genomic DNA, endotoxin, open circular (oc) and linear pDNA isoforms, resulting in the isolation of the supercoiled (sc) pDNA isoform.

Your benefits:

• Precision - Produces low endotoxin, highly homogeneous supercoiled pDNA of clinical grade.
• Scalability - Applicable for bench scale purification and can be easily scaled up for manufacturing of pDNA as raw material or drug substance.
• Efficiency - The advantages of monolithic columns allow high flow rates and short processing times.