CIMac™ C4 HLD 0.1 mL Analytical Column (2 µm)
CIMac™ Analytical Columns combine all elaborate characteristics of CIM® monoliths at analytical scale and are your perfect tool for analysis and control of manufacturing processes, ideal for process analytical technology (PAT).
Designed for fast and reproducible HPLC monitoring and quantitation of proteins, viruses, pDNA, and other large biomolecules, they allow you to achieve rapid, high-resolution and flow-independent separations in a matter of minutes.
- Item No.:
- 110.8136-2
*Custom/bulk order quotes are provided within 72 hours of request.
Choose your CIMac™
CIMac™ Analytical Columns are short-bed high-performance monolithic columns offering all the advantages of CIM® monolithic technology. Their small volume and short length enable high volumetric flow rates, allowing for the rapid acquisition of quantity and purity information within minutes.
Hence, the CIMac™ Analytical Columns are ideal for the in-process and final control of various samples from different purification process steps.
Features:
- Volume of 0.1 mL (0.3 mL for CIMac™ pDNA)
- Flow rates from 1 to 30 column volumes/minute (varies by type)
- Application-optimized channel widths
- Available in a wide variety of chemistries
Benefits:
- Analytical HPLC columns for nucleic acids, viral vectors, viruses, and other biomolecules
- In-process analysis and quantitation, and final product
- At-line monitoring and control of manufacturing processes (PAT)
- Rapid, high-resolution analysis achieved in minutes
- High sensitivity, accuracy and reproducibility
- No sample carryover
Applications
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ApplicationAnalytical Columns
Connections (Physical)
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Connector(s)10-32 UNF coned port, 0.0625" OD tubing connection
Dimensions
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Channel Size2 µm
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Diameter5.2 mm
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Length4.95 mm
Features
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ReusableYes
General Specifications
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Flow Rate0.2 – 3 mL/min | 1 – 15 cm/min | 2 – 30 CV/min
Materials of Construction
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Materials of ConstructionPoly(butyl methacrylate -co- ethylene dimethacrylate)
Operating Conditions
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Maximum Operating Pressure15 MPa | 150 bar | 2175 psi
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Recommended Operating Temperature2 °C (36 °F) to 25 °C (77 °F)
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pH StabilityWorking range 3–13, cleaning in place 1–14
Physicochemical Information
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Bed Volume0,1 mL
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LigandC4 HLD (hydrophobic, butyl – high ligand density)
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Typical Dynamic Binding Capacity 10% per mL2.5–3.5 mg pEGFP/mL wet support, pEGFP (4.7 kbp) 0.04 mg/mL, 50 mM TRIS, 10 mMEDTA, 3 M (NH₄)₂SO₄, pH 7.2
Product Information
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BrandCIMac™
Biocompatible analytical chromatography system with quaternary buffer setup.
PATfix® analytical quaternary system is an at-line tool, to help process developers of novel gene therapies get immediate feedback on their current process state. This enables faster iteration and process optimization towards better purities and higher recoveries. The Sartorius analytical system provides modality-appropriate analysis and in-process control for gene therapy and vaccine products. Designed to take full advantage of the capabilities of CIMac™ analytical monolithic columns, the PATfix® delivers fast, high-resolution runs. Operation with multiple detector types provides content- rich analyses even with complex samples. User friendly PATfix® software makes for easy data processing, visualization and sharing.