CIMac™ EDA 0.1 mL Analytical Column (1.3 µm)
This high-performance ion-exchange column is primarily intended for fast, efficient and reproducible analysis and in-process control of samples throughout different purification steps.
- Item No.:
- 110.5116-1.3
- Piece
- 1
*Custom/bulk order quotes are provided within 72 hours of request.
Choose your CIMac™
CIMac™ Analytical Columns are intended for fast and reproducible HPLC monitoring and quantitation of large proteins, plasmid DNA, viruses, virus-like particles, and phages. They were primarily designed for the in-process and final control of various samples at different stages of the purification process. They can be operated at high volumetric flow rates (from 1 to 20 column volumes/min); therefore, the information about the product quantity and purity can be obtained within a few minutes. CIMac™ Analytical Columns are fast and reliable PAT tools, providing:
- Rapid, high resolution analysis, achieved in minutes
- Flow independent separation
- High sensitivity and reproducibility
EDA (ethylenediamine) features:
- Weak anion exchanger
- Working range, pH 2–9
- Binds molecules with predominantly negative charge, repels molecules a predominantly positive charge
- Use for capture, intermediate, or polishing steps in either bind-elute or flow-through mode
- Sanitizable with 1 M NaOH
Applications
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ApplicationAnalytical Columns
Connections (Physical)
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Connector(s)10-32 UNF coned port, 0.0625" OD tubing connection
Dimensions
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Channel Size1.3 µm
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Diameter5.2 mm
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Length4.95 mm
Features
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ReusableYes
General Specifications
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Flow Rate0.2 – 3 mL/min | 1 – 15 cm/min | 2 – 30 CV/min
Materials of Construction
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Materials of ConstructionPoly(glycidyl methacrylate -co- ethylene dimethacrylate)
Operating Conditions
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Maximum Operating Pressure15 MPa | 150 bar | 2175 psi
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Recommended Operating Temperature4 °C (39 °F) to 40 °C (104 °F)
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pH StabilityWorking range 2–10, cleaning in place 1–14
Physicochemical Information
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Bed Volume0.1 mL
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LigandEDA (weak anion exchanger, ethylene diamine)
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Ligand Density0.7–1.1 mmol/mL wet support
Product Information
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BrandCIMac™
Biocompatible analytical chromatography system with quaternary buffer setup.
PATfix® analytical quaternary system is an at-line tool, to help process developers of novel gene therapies get immediate feedback on their current process state. This enables faster iteration and process optimization towards better purities and higher recoveries. The Sartorius analytical system provides modality-appropriate analysis and in-process control for gene therapy and vaccine products. Designed to take full advantage of the capabilities of CIMac™ analytical monolithic columns, the PATfix® delivers fast, high-resolution runs. Operation with multiple detector types provides content- rich analyses even with complex samples. User friendly PATfix® software makes for easy data processing, visualization and sharing.