CIMac™ pDNA 0.3mL Analytical Column (1.4 µm)
CIMac™ pDNA is an excellent choice for fast and reproducible monitoring and quantification of plasmid DNA. It is especially useful for separation of different plasmid DNA isoforms.
- Item No.:
- 150.8501-1.4
- Piece
- 1
*Custom/bulk order quotes are provided within 72 hours of request.
Choose your CIMac™
CIMac™ Analytical Columns are short-bed high-performance monolithic columns offering all the advantages of CIM® monolithic technology. Their small volume and short column length allow the operation at high volumetric flow rates, enabling them to receive the information about the product quantity and purity in just a few minutes. Hence, the CIMac™ Analytical Columns can be effectively used for the in-process and final control of various samples from different purification process steps.
The CIMac™ pDNA-0.3 Analytical Columns are intended for fast and reproducible HPLC monitoring and quantitation of plasmid DNA and are able to separate different plasmid DNA isoforms. They were primarily designed for the in-process and final control of pDNA samples at different stages of the purification process. They can be operated at high volumetric flow rates (from 1 to 10 column volumes/min); therefore, the information about the pDNA quantity and purity can be obtained within a few minutes.
CIMac™ pDNA-0.3 Analytical Columns are fast and reliable PAT tools, providing:
- Rapid, high resolution analysis, achieved in minutes
- Flow independent separation
- High sensitivity and reproducibility
pDNA features:
- Weak anion exchanger
- Enables fast and reproducible monitoring and quantitation of pDNA purification, and high resolution separation of DNA plasmid isoforms
Applications
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ApplicationAnalytical Columns
Connections (Physical)
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Connector(s)10-32 UNF coned port, 0.0625" OD tubing connection
Dimensions
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Channel Size1.4 µm
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Diameter5.2 mm
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Length15 mm
Features
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ReusableYes
General Specifications
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Flow Rate0.2 – 3 mL/min | 1 – 15 cm/min | 0.67 – 10 CV/min
Materials of Construction
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Materials of ConstructionPoly(glycidyl methacrylate -co- ethylene dimethacrylate)
Operating Conditions
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Maximum Operating Pressure10 MPa | 100 bar | 1450 psi
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Recommended Operating Temperature4 °C (39 °F) to 40 °C (104 °F)
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pH StabilityWorking range 3–9, cleaning in place 1–14
Physicochemical Information
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Bed Volume0.3 mL
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LigandDEAE (weak anion exchanger, diethylamino)
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Ligand Density1.0–1.15 mmol/mL wet monolith
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Typical Dynamic Binding Capacity 10% per mL21–25 mg BSA/mL wet support, BSA, 1.0 mg/mL, 20 mM Tris-HCl buffer, pH 7.4, flow rate1 mL/min
Product Information
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BrandCIMac™
Biocompatible analytical chromatography system with quaternary buffer setup.
PATfix® analytical quaternary system is an at-line tool, to help process developers of novel gene therapies get immediate feedback on their current process state. This enables faster iteration and process optimization towards better purities and higher recoveries. The Sartorius analytical system provides modality-appropriate analysis and in-process control for gene therapy and vaccine products. Designed to take full advantage of the capabilities of CIMac™ analytical monolithic columns, the PATfix® delivers fast, high-resolution runs. Operation with multiple detector types provides content- rich analyses even with complex samples. User friendly PATfix® software makes for easy data processing, visualization and sharing.
Rapid at-line analytical chromatography solution for all stages of pDNA processing.
PATfix® pDNA is a biocompatible platform, engineered for pDNA isoform and multimer separation. Built around analytical capability of the CIMac® pDNA monolithic columns, the pDNA platform includes user guides, validated methods, SOPs and pDNA standards and user-friendly PATfix® software.
CIMac™ pDNA is an excellent choice for fast and reproducible monitoring and quantification of plasmid DNA. It is especially useful for separation of different plasmid DNA isoforms.