CIMac™ r-Protein A 0.1 mL Analytical Column (2 µm)
CIMac™ r-Protein Analytical Columns are primarily intended for fast, efficient, and reproducible analysis of immunoglobulin G (IgG).
- Item No.:
- 110.1004-2
- Piece
- 1
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CIMac™ Analytical Columns are short-bed high-performance monolithic columns offering all the advantages of CIM® monolithic technology. Their small volume and short column length allow the operation at high volumetric flow rates, enabling them to receive the information about the product quantity and purity in just a few minutes. Hence, the CIMac™ Analytical Columns can be effectively used for the in-process and final control of various samples from different purification process steps.
CIMac™ r-Protein Analytical Columns are primarily intended for fast, efficient, and reproducible analysis of immunoglobulin G (IgG) from various samples. They can be effectively used for in-process and final control (quantitative and qualitative) of IgG purified from cell culture supernatant or human plasma. The innovative stainless steel housing allows flow in both directions, increasing its durability and ease of use.
rProtein A (recombinant protein A from E. coli) features:
- Used for purification of mouse and most subtypes of human immunoglobulin G (IgG)
- Primary binding to the hinge region of IgG-Fc of IgG, secondary binding to light chains of other antibody classes
- Most suitable for purification of human IgG, and many IgM monoclonal antibodies
Applications
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ApplicationAnalytical Columns
Connections (Physical)
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Connector(s)10-32 UNF coned port, 0.0625" OD tubing connection
Dimensions
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Channel Size2 µm
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Diameter5.2 mm
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Length4.95 mm
Features
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ReusableYes
General Specifications
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Flow Rate0.2 – 3 mL/min | 1 – 15 cm/min | 2 – 30 CV/min
Materials of Construction
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Materials of ConstructionPoly(glycidyl methacrylate -co- ethylene dimethacrylate)
Operating Conditions
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Maximum Operating Pressure15 MPa | 150 bar | 2175 psi
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Recommended Operating Temperature4 °C (39 °F) to 40 °C (104 °F)
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pH StabilityWorking range 2–11, cleaning in place 2–13
Physicochemical Information
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Bed Volume0.1 mL
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Ligandr-Protein A (affinity, recombinant protein A produced from E. Coli coupled to CDI-preactivated support)
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Typical Dynamic Binding Capacity 10% per mL≥ 5 mg hIgG/mL wet support, human IgG 0.5 mg/mL, PBS buffer, pH 7.4, flow rate 1mL/min, Human IgG: octagam® 5% (Octapharma®)
Product Information
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BrandCIMac™
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