CIMac™ XY 0.1 mL Analytical Column (1.3 µm)
Epoxy monoliths are used for covalent immobilisation of peptides, proteins and enzymes containing a reactive nucleophilic group (e.g. amino, thiol, hydroxyl) on their surface.
- Item No.:
- 110.7175-1.3
*Custom/bulk order quotes are provided within 72 hours of request.
Choose your CIMac™
CIMac™ Analytical Columns are short-bed high-performance monolithic columns offering all the advantages of CIM® monolithic technology. Their small volume and short column length allow the operation at high volumetric flow rates, enabling them to receive the information about the product quantity and purity in just a few minutes. Hence, the CIMac™ Analytical Columns can be effectively used for the in-process and final control of various samples from different purification process steps
Epoxy monoliths can be used for covalent immobilisation of peptides, proteins and enzymes containing a reactive nucleophilic group (e.g. amino, thiol, hydroxyl) on their surface. The covalent nature of the bond between the ligand and matrix reduces leaching and improves stability and reusability. Immobilised supports enable a variety of customised analytical affinity chromatography options with interactions specific to the target molecule.
XY (epoxy) features:
- Forms a covalent epoxy linkage with amino and thiol-containing molecules
- Suitable for coupling of sensitive molecules, such as peptides, proteins, oligonucleotides
Applications
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ApplicationAnalytical Columns
Connections (Physical)
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Connector(s)10-32 UNF coned port, 0.0625" OD tubing connection
Dimensions
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Channel Size1.3 µm
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Diameter5.2 mm
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Length4.95 mm
Features
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ReusableYes
General Specifications
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Flow Rate0.2 – 3 mL/min | 1 – 15 cm/min | 2 – 30 CV/min
Materials of Construction
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Materials of ConstructionPoly(glycidyl methacrylate -co- ethylene dimethacrylate)
Operating Conditions
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Maximum Operating Pressure15 MPa | 150 bar | 2175 psi
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Recommended Operating Temperature2 °C (36 °F) to 8 °C (46 °F)
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pH StabilityWorking range 1–14
Physicochemical Information
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Bed Volume0,1 mL
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LigandXY (activated; epoxy)
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Ligand Density1.6 mmol/mL wet support
Product Information
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BrandCIMac™
Biocompatible analytical chromatography system with quaternary buffer setup.
PATfix® analytical quaternary system is an at-line tool, to help process developers of novel gene therapies get immediate feedback on their current process state. This enables faster iteration and process optimization towards better purities and higher recoveries. The Sartorius analytical system provides modality-appropriate analysis and in-process control for gene therapy and vaccine products. Designed to take full advantage of the capabilities of CIMac™ analytical monolithic columns, the PATfix® delivers fast, high-resolution runs. Operation with multiple detector types provides content- rich analyses even with complex samples. User friendly PATfix® software makes for easy data processing, visualization and sharing.