HA Ultrogel® - 100 mL

Tech Support Documents
HA Ultrogel® - 100 mL HA Ultrogel® - 100 mL
 

HA Ultrogel® is a composite material of cross-linked agarose and microcrystalline hydroxyapatite enclosed in the agarose matrix. The material shows mixed mode functionality based on cation exchange and metal affinity in the hydroxyapatite structure. A wide variety of applications include separation of proteins, peptides and nucleic acids, from laboratory to production scale.

Pack Volume
Price (USD):
$386.00
Item No.: 
24775-025
Availability: 

*Custom/bulk order quotes are provided within 72 hours of request.

HA Ultrogel® resin is a hydroxyapatite agarose composite resin for the separation of biomolecules from research and to manufacturing scale. Hydroxyapatite chromatography is considered to be a “pseudo-affinity” chromatography, or “mixed-mode” ion exchange. It has proven to be an effective purification mechanism in a variety of processes, providing biomolecule selectivity complementary to more traditional ion exchange or hydrophobic interaction techniques.

Features and Benefits

- Unique selectivity and separation mechanism, complementary to traditional IEX and HIC
- Protein purification in neutral, non-denaturating conditions
- High porosity, easy cleaning

Applications

- Purification of human serum proteins
- Purification of basic proteins, of phosphate-dependent proteins and enzymes, and of DNA-dependent enzymes
- Vaccine purification processes, e.g. Bordetella pertussis toxin
- Separation of plant proteins such as lectins, glycoproteins, glycosidases, phospholipidases, sulfohydrolases, sphingomyelinases, transferases, trehalases and kinases

Applications

  • Application
    Preparative protein purification

Compliance Information

  • Quality Standards
    • CoA
    • RSF

Dimensions

  • Bead Size
    120 µm

General Specifications

  • Flow Rate
    < 100 cm/h
  • Resin Format
    Bulk resin

Materials of Construction

  • Base Matrix
    Agarose

Operating Conditions

  • Operating Pressure
    < 3 barg (44psi)

Physicochemical Information

  • Ligand
    Hydroxyapatite
  • Reference Protein
    Cytochrome C
  • Typical Dynamic Binding Capacity 10% per mL
    > 7 mg/mL Cytochrome C

Product Information

  • Brand
    HA Ultrogel®
  • Pack Size
    1
  • Pack Volume
    100 mL
  • Resin Delivery Condition
    1M NaCl with 20% EtOH
  • Resin Type
    Mixed-mode cation exchanger
  • What you receive
    1 bottle resin, instructions

Storage Conditions

  • Storage Conditions
    20% EtOH/1M NaCl