iQue® Human T Cell Exhaustion Kit

The Human T Cell Exhaustion Cell and Cytokine Profiling Kit is designed for ease of use in multiplexing markers of T cell exhaustion, phenotyping T helper and T cytotoxic cells, and bead-based measurement of secreted cytokines, all in the same assay. This optimized assay offers these unique advantages:

• Live immune cells are distinguished from dead or membrane compromised cells by staining with a fluorescent membrane integrity dye.

• Cells are immunophenotyped by staining with a fluorescent antibody panel to distinguish CD3+ T cells, CD3- non-T cells, CD3+CD4+ helper T cells, and CD3+CD8+ cytotoxic T cells.

• The panel also includes three markers of T cell exhaustion: PD-1, Tim-3, and Lag-3.

• Optimized workflow enables the measurement of cytokines secreted by activated T cells (IFNγ and TNF) in the same assay well.

• The capacity of the sample cells to proliferate can also be determined with an optional proliferation dye if desired (included with the kit).

Note: This product is not returnable. If you have any issues with this product, please contact your Sartorius sales representative for assistance.